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Plasmid#175292PurposeVisualize endogeneous loci coupled with ZF probes harboring 10xHA tagDepositorInsertanti-HA frankenbody fused with mCherry
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-U6-3-t-attP40
Plasmid#133561PurposegRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructsDepositorInsertattP40 region guide RNAs
UseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-Uricase
Plasmid#182401PurposeBacterial expression of Vesicle Nucleating peptide-uricase fusionDepositorInsertVNp-uricase
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIVT/Smarca4
Plasmid#122146PurposeFor making Smarca4 RNADepositorInsertSmarca4 (Smarca4 Mouse)
UseFor in vitro transcriptionTagsExpressionMutationPromoterT7Available sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-Nluc-2xC/D box
Plasmid#180504PurposeMammalian expression of Nluc-2xC/D boxDepositorInsertNluc-2xC/D box
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF2703
Plasmid#144151PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertESRRA (ESRRA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
ECFP HO
Plasmid#207413PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertECFP
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
ECFP CD
Plasmid#207414PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertECFP
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_Puro
Plasmid#167863PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SCov2-nsp7
Plasmid#154757PurposeExpression of SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGIP
Plasmid#26777PurposeLentiviral vector for stable integration of GFP expression cassette with puromycin selection.DepositorInsertEF1a-GFP-IRES-Puro (gfp )
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
CTNNB1 in pENTR
Plasmid#216617Purposegateway cloning donor vector containing CTNNB1DepositorInsertCTNNB1 (CTNNB1 Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
mApple-pBAD
Plasmid#54536PurposeLocalization: Protein Expression Vector , Excitation: 568, Emission: 592DepositorTypeEmpty backboneUseTags6xHis and mAppleExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
p006-Banana-Late
Plasmid#112251PurposeATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.DepositorInsertATF1 under osmY promoter
UseTagsExpressionBacterialMutationPromoterosmYAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
VN-Tau (wt)
Plasmid#87368Purposeexpresses hTau fused to the N-terminal part of VenusDepositorInsertMAPT (MAPT Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGW-GOI-TurboID
Plasmid#209378PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK1
Plasmid#111722Purposeto express human BLM protein in yeast cellsDepositorInsertBLM (BLM Human)
UseTagshexahistidineExpressionYeastMutationa C-terminal hexahistidine tag was added, a yeast…PromoterGAL1Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU40
Plasmid#173831PurposeExpresses 40 kD Penn State ladder proteinDepositorInsertHSTPABPACGST
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42-Q61L
Plasmid#12986DepositorInsertCdc42 constitutively active (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationQ61L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only