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Plasmid#170808PurposePiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistanceDepositorInsertsUseTagsExpressionMammalianMutationSynonymous mutation in PYL1 to remove XhoI site (…PromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only
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pTT5-hSDC1-CS1
Plasmid#52365Purposeexpresses human Syndecan-1 point mutant at S184A in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationR95Q and S184A (according to numbering in Fig. 2A…PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
DCLK1 gRNA (BRDN0001146493)
Plasmid#76748Purpose3rd generation lentiviral gRNA plasmid targeting human DCLK1DepositorInsertDCLK1 (DCLK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
DCLK1 gRNA (BRDN0001147487)
Plasmid#76747Purpose3rd generation lentiviral gRNA plasmid targeting human DCLK1DepositorInsertDCLK1 (DCLK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF086 MBP-CREB 127-135-His
Plasmid#154075PurposeExpresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCREB1 (CREB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
DCLK1 gRNA (BRDN0001145902)
Plasmid#76746Purpose3rd generation lentiviral gRNA plasmid targeting human DCLK1DepositorInsertDCLK1 (DCLK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS
Plasmid#85821PurposeiDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertZf Ubi::cMyc oDam_f_GFPoNLS
UseTol2 based vectorTagscMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…ExpressionMutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-S15A
Plasmid#52363Purposeexpresses human Syndecan-1 serine 15 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationS15A and R95Q (according to numbering in Fig. 2A …PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 S387E
Plasmid#59906PurposeExpresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationS387E, phospho-dominantPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcs2-ATF4uORF1-2-GFP-IRES-mCherry
Plasmid#226105PurposeATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)DepositorInsertatf4 (ATF4 Human)
UseTagsGFPExpressionMammalianMutationcontains uORF1-2 of ATF4, whose stability can ser…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE FL NAP1 STOP C' EGFP IRES Blast
Plasmid#179102PurposeExpresses untagged full length NAP1DepositorInsertNak associated protein 1 (AZI2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-N'-FLAG-HA-NAP1S318A
Plasmid#195410PurposeExpresses FLAG-HA tagged human NAP1 with S318A point mutationDepositorInsertNak associated protein 1 (AZI2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationSerine 318 turned to AlaninePromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-NLSmCherryNLS Clover-HDHB
Plasmid#190223PurposeFluorescent cell cycle reporterDepositorInsertsUseTransposonTagsClover fluorescent proteinExpressionMammalianMutationAmino Acids 994-1087PromoterEF1a and RPBSAAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hPEN2 2A
Plasmid#183640PurposeLentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).DepositorInsertPEN2 (PSENEN Human)
UseLentiviralTagsHAExpressionMutationresidues 35 and 40 of PEN2 mutated to alaninePromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PMP34-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170997PurposeHiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)DepositorInsertPMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-JIP60ml
Plasmid#104959PurposeEncodes a modified, active, form of Hordeum vulgare c.v. Golden Promise Jasmonate Induced Protein 60 (JIP60)DepositorInsertJasmonate Induced Protein 60 (JIP60)
UseGateway entry vector for lr recombination into ot…TagsExpressionMutationReplaced amino acids 164 to 187 with a methionine…PromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSSO2462
Plasmid#97064PurposeExpressing Sulfolobus solfataricus orf SSO2462 proteinDepositorInsertS. solfataricus orf SSO2462
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAMK1G gRNA (BRDN0001146460)
Plasmid#75986Purpose3rd generation lentiviral gRNA plasmid targeting human CAMK1GDepositorInsertCAMK1G (CAMK1G Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAMK1G gRNA (BRDN0001148530)
Plasmid#75984Purpose3rd generation lentiviral gRNA plasmid targeting human CAMK1GDepositorInsertCAMK1G (CAMK1G Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAMK1G gRNA (BRDN0001147776)
Plasmid#75985Purpose3rd generation lentiviral gRNA plasmid targeting human CAMK1GDepositorInsertCAMK1G (CAMK1G Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only