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We narrowed to 38 results for: LIC

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Showing: 1 - 20 of 38 results

  1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...Ligation-independent cloning Ligation-independent cloning (LIC) was first developed in the 1990s. While traditional...transformation, so the cloned vector must be ligated. But in LIC, longer single-stranded overhangs can hold a nicked...repairable by the bacteria after transformation. LIC is a reliable cloning method, but it is limited by...back the entire 10–12 bases. As such, the use of LIC is often limited to specially designed plasmids. ...ligation-independent cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene...-independent cloning (SLIC), eliminated many of LIC’s constraints by relying on the power of homologous...

  4. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...26094 pET28a-LIC EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag...cleavage, KanR 26098 pCW-LIC EF460848 No tag, double ptac promoter, AmpR 26099 pFBOH-LIC EF456740 Hexahistidine...AmpR 26108 pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression... Bac-to-Bac Expression System, AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with...

  5. Ligation Independent Cloning

    Type
    Protocol
    ... as an example of LIC experimental design. Search Addgene's collection of empty LIC cloning vectors Protocol...Protocols Overview Ligation Independent Cloning (LIC) obviates the need for the time-consuming ligation... through the transformation/replication process. LIC employs long overhangs to form a stable association... during the replication cycle. Empty vectors for LIC typically employ type II restriction enzymes (e.g...in the example shown here. The manufacturer of an LIC-specific vector will provide the homologous sequence...Protocol Step 1: Design Your Primers Primer design for LIC is often as simple as using the backbone manufacturer's... protocol . Search Addgene's collection of empty LIC cloning vectors...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... promoter pFastBac LIC and pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression...cells His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent...mammalian expression, Ligation Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression ...bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression collection page...Trx (thioredoxin) Protein purification pNH-TrxT - LIC cloning in bacteria with N-terminal His-TrxA-TEV ...

  9. Molecular Cloning Techniques

    Type
    Guide
    ...Independent Cloning (LIC) relies on the 3' to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA ...nicks that bacteria repair after transformation. LIC does not require site-specific recombination or a...making it an easy, cheap, and rapid cloning method. LIC depends on the addition of only one free dNTP to ...will repair the nicks. It is important to note that LIC has difficulty assembling DNA fragments with repetitive...more in our SLIC blog post . Figure 6: Summary of LIC. Created with BioRender.com. Yeast-mediated Cloning...

  13. TALEN Plasmids and Kits

    Type
    Collection
    .... Feng Zhang LIC TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in...
Showing: 1 - 20 of 38 results