pHAGE FL NAP1 STOP C' EGFP IRES Blast
(Plasmid
#179102)
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PurposeExpresses untagged full length NAP1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179102 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHAGE-C-EGFP-GAW-BLAST
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Backbone manufacturerJ. Wade Harper's Laboratory
- Backbone size w/o insert (bp) 8293
- Total vector size (bp) 9473
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNak associated protein 1
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SpeciesH. sapiens (human)
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Insert Size (bp)1179
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Entrez GeneAZI2 (a.k.a. AZ2, NAP1, TILP)
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMVf
- 3′ sequencing primer EGFPN1R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE FL NAP1 STOP C' EGFP IRES Blast was a gift from Alicia Pickrell (Addgene plasmid # 179102 ; http://n2t.net/addgene:179102 ; RRID:Addgene_179102) -
For your References section:
NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis. Paul S, Sarraf SA, Nam KH, Zavar L, DeFoor N, Biswas SR, Fritsch LE, Yaron TM, Johnson JL, Huntsman EM, Cantley LC, Ordureau A, Pickrell AM. J Cell Biol. 2024 Feb 5;223(2):e202303082. doi: 10.1083/jcb.202303082. Epub 2023 Dec 7. 10.1083/jcb.202303082 PubMed 38059900
Map uploaded by the depositor.
