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Plasmid#184645PurposeRetroviral expression of BirA Rap1 I312RDepositorInsertBirA-rap1-I312R
UseRetroviralTagsBirA FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8319 pDONR ATG-YAP1 WT-Stop
Plasmid#184524PurposeSubcloning YAP1 WTDepositorInsertYAP1 WT (YAP1 Human)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-CfANLN_sgRNA
Plasmid#183879PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.DepositorInsertCanis familiaris ANLN sgRNA spacer
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-H2BC11_sgRNA
Plasmid#183883PurposepX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.DepositorInsertH2BC11 sgRNA spacer (H2BC11 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-MYH10_sgRNA
Plasmid#183887PurposepX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.DepositorInsertMYH10 sgRNA spacer (MYH10 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-H2BC11_sgRNA
Plasmid#183881PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.DepositorInsertH2BC11 sgRNA spacer (H2BC11 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-MYH10_sgRNA
Plasmid#183886PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.DepositorInsertMYH10 sgRNA spacer (MYH10 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-MNTS-1-p75
Plasmid#182945PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTRDepositorUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV promoter and enhancerAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-MNTS-1
Plasmid#182944PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTRDepositorInsertMNTS-1 (NTF3 Human, Homo sapiens, Synthetic)
UseLentiviralTagsExpressionMammalianMutationThis is the Human NT-3 with mutations on the matu…PromoterCMV promoter and enhancerAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-DUXB-CA
Plasmid#125027PurposeAll-in-one lentivector with tet-inducible DUXB (codon sequences altered)DepositorInsertDUXB (codon altered) (DUXB Human)
UseLentiviralTagsExpressionMammalianMutationcodon alteredPromoterTREAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry
Plasmid#182381PurposeDual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promotersDepositorInsertsUseTags3xFlagExpressionMammalianMutationChanged CDS sequence from 'ccacgttcaagtcacga…PromoterHSV TK promoter and SV40 promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-RB_B
Plasmid#145977PurposeInsect Expression of DmAGO1-RBDepositorInsertDmAGO1-RB (AGO1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA1-gltA2
Plasmid#71348PurposegltA1-gltA2 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA1-gltA2 guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET Duet1 His6 SUMO D14
Plasmid#177854PurposeBacterial Expression of D14DepositorInsertD14
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His-GBAi W211A
Plasmid#171756PurposeFor the expression of His-GBAi W211A in bacteriaDepositorInsertGBAi
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-bPAC(R278A)-Myc-eYFP
Plasmid#165488PurposeXenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activityDepositorInsertbPAC(R278A)-Myc-eYFP
UseCdna expression, xenopus oocyteTagsEYFP and MycExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SPN_ARR N52R (Shank3)
Plasmid#175255PurposeSPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expressionDepositorInsertSPN-ARR fragment with N52R mutation from SHANK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTF101.1gw3
Plasmid#172181PurposeA binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorInsertattR3-CmR-ccdB-attR4
UseTagsExpressionPlantMutationPromoterlacUV5 promoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-myc-BioID-hp100
Plasmid#174738PurposeGene overexpressionDepositorInsertP100 (NFKB2 Human)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-5.3-GFP-HiBit
Plasmid#171014PurposeFor bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-5.3-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only