Skip to main content
Addgene

pLV-MNTS-1-p75
(Plasmid #182945)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182945 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLV-MP2
  • Backbone manufacturer
    Pantelis Tsoulfas
  • Backbone size w/o insert (bp) 6756
  • Total vector size (bp) 7533
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    If does not grow well use low sodium LB.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MNTS-1-p75
  • Alt name
    NT-3
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    777
  • Entrez Gene
    NTF3 (a.k.a. HDNF, NGF-2, NGF2, NT-3, NT3)
  • Entrez Gene
    NT3
  • Promoter CMV promoter and enhancer

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-MNTS-1-p75 was a gift from Pantelis Tsoulfas (Addgene plasmid # 182945 ; http://n2t.net/addgene:182945 ; RRID:Addgene_182945)
  • For your References section:

    The binding epitopes of neurotrophin-3 to its receptors trkC and gp75 and the design of a multifunctional human neurotrophin. Urfer R, Tsoulfas P, Soppet D, Escandón E, Parada LF, Presta LG. EMBO J. 1994 Dec 15. 13(24):5896-909. PubMed 7529173