pLV-MNTS-1
(Plasmid
#182944)
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PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTR
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-MNTS1
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Backbone manufacturerPantelis Tsoulfas
- Backbone size w/o insert (bp) 6759
- Total vector size (bp) 7536
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsIf does not grow well use low sodium LB.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMNTS-1
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Alt nameNT-3
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SpeciesH. sapiens (human), Synthetic; Homo sapiens
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Insert Size (bp)777
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MutationThis is the Human NT-3 with mutations on the mature protein at position D15 and six amino acids from the N-terminal sequence of NGF
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Entrez GeneNTF3 (a.k.a. HDNF, NGF-2, NGF2, NT-3, NT3)
- Promoter CMV promoter and enhancer
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-MNTS-1 was a gift from Pantelis Tsoulfas (Addgene plasmid # 182944 ; http://n2t.net/addgene:182944 ; RRID:Addgene_182944) -
For your References section:
The binding epitopes of neurotrophin-3 to its receptors trkC and gp75 and the design of a multifunctional human neurotrophin. Urfer R, Tsoulfas P, Soppet D, Escandón E, Parada LF, Presta LG. EMBO J. 1994 Dec 15. 13(24):5896-909. PubMed 7529173