pCW57.1-DUXB-CA
(Plasmid #125027)

• Purpose: All-in-one lentivector with tet-inducible DUXB (codon sequences altered)
• Depositing Lab: Stephen Tapscott
• Publication: Chew et al Dev Cell. 2019 Sep 9;50(5):658-671.e7. doi: 10.1016/j.devcel.2019.06.011. Epub 2019 Jul 18.

Backbone

• Vector backbone: pCW57.1
• Backbone size w/o insert (bp): 9354
• Total vector size (bp): 8819
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: We used Stbl3 cells (recombination deficient)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DUXB (codon altered)
• Species: H. sapiens (human)
• Insert Size (bp): 1038
• Mutation: codon altered
• GenBank ID: NM_001351307
• Entrez Gene: DUXB
• Promoter: TRE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: tcgcctggagaattggc
• 3′ sequencing primer: agggctgccttggaaaag

Resource Information

• Supplemental Documents:
  • pCW57.1_DUXB_Codon_altered.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry