159,371 results

1. Lenti-TurboCas-NLS-Blast

   Plasmid

   #232346

   Purpose

   Lentiviral expression vector for dCas9 fused to miniTurboNLS

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA, miniTurbo, V5 and 3xSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1a-hMLH1dn

   Plasmid

   #174824

   Purpose

   Mammalian expression of human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (codon optimized)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. rAAV2-retro helper

   Plasmid

   #81070

   Purpose

   retrograde AAV helper vector, carrying rep and modified capsid gene

   Depositor

   Alla Karpova, David Schaffer

   Article

   Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

   Inserts

   rep
   rAAV2-retro cap
   

   Use

   AAV and Unspecified; Helper vector

   Tags

   Expression

   Mutation

   Pro2Ala, possibly Asp613Gly and changes made to A…

   Promoter

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRY2-mCherry-Miro1TM

   Plasmid

   #102247

   Purpose

   Expresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   CRY2PHR-mCherry-Miro1TM (RHOT1, CRY2 Mustard Weed, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Mito-7

   Plasmid

   #55102

   Purpose

   Localization: Mitochondria, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. NanoLuc-PM-Venus

   Plasmid

   #164786

   Purpose

   Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
   

   Use

   Tags

   NanoLuc and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry- SEC61B

   Plasmid

   #121160

   Purpose

   Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FgH1tUTG

   Plasmid

   #70183

   Purpose

   Inducible expression of guide RNA with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   H1-Tet-sgrna cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLCAR-CD19-BBz

   Plasmid

   #135992

   Purpose

   Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-puro-hTERT

    Plasmid

    #1771

    Purpose

    Retroviral expression of hTERT. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3-EF1aH

    Plasmid

    #180149

    Purpose

    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-MAPPER

    Plasmid

    #117721

    Purpose

    MAPPER Selectively Labels ER-PM Junctions

    Depositor

    Jen Liou

    Article

    Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

    Insert

    MAPPER
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR-A-HYG

    Plasmid

    #29635

    Depositor

    Scott Gold, David_L Andrews

    Article

    Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

    Type

    Empty backbone

    Use

    Gateway donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pemiRFP670-N1

    Plasmid

    #136556

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-LC3

    Plasmid

    #11546

    Depositor

    Karla Kirkegaard

    Article

    Jackson et al PLoS Biol. 2005 May . 3(5):e156.

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-iCas9-neo

    Plasmid

    #85400

    Purpose

    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Insert

    Flag-iCas9-P2A-GFP
    

    Use

    Lentiviral

    Tags

    Flag-iCas9-P2A-GFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-KRAB

    Plasmid

    #110820

    Purpose

    Conventional dCas9 repressor-dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. FUCas9Cherry

    Plasmid

    #70182

    Purpose

    Expresses Cas9 with fluorescent Cherry reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    humanised S.pyogenes cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-EGFP

    Plasmid

    #13031

    Purpose

    Mammalian expression vector for cloning your gene fused to EGFP

    Depositor

    Doug Golenbock

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCX420

    Plasmid

    #229211

    Purpose

    Cytoplasmic-targeting Csm complex plasmid

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    Csm1; Csm2; Csm3(RNase mut)-2xsfGFP; Csm4; Csm5; Cas6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge