Showing: 1181 - 1200 of 144942 results
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Plasmid#59841PurposeConstitutively expresses AmiE with proK6 promoterDepositorInsertALIPHATIC AMIDASE (amiE )
UseTags6x-HisExpressionBacterialMutationPromoterproK6AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK3_amiE
Plasmid#59842PurposeConstitutively expresses AmiE with proK3 promoterDepositorInsertALIPHATIC AMIDASE (amiE )
UseTags6x-HisExpressionBacterialMutationPromoterproK3AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK1_amiE
Plasmid#59843PurposeConstitutively expresses AmiE with proK1 promoterDepositorInsertALIPHATIC AMIDASE (amiE )
UseTags6x-HisExpressionBacterialMutationPromoterproK1AvailabilityAcademic Institutions and Nonprofits only -
pJK_pro6_amiE
Plasmid#59844PurposeConstitutively expresses AmiE with pro6 promoterDepositorInsertALIPHATIC AMIDASE (amiE )
UseTags6x-HisExpressionBacterialMutationPromoterpro6AvailabilityAcademic Institutions and Nonprofits only -
pJK_proB_eGFP
Plasmid#59845PurposeConstitutively expresses eGFP with proB promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproBAvailabilityAcademic Institutions and Nonprofits only -
pJK_proK16_eGFP
Plasmid#59847PurposeConstitutively expresses eGFP with proK16 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK16AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK14_eGFP
Plasmid#59848PurposeConstitutively expresses eGFP with proK14 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK14AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK9_eGFP
Plasmid#59849PurposeConstitutively expresses eGFP with proK9 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK9AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK6_eGFP
Plasmid#59850PurposeConstitutively expresses eGFP with proK6 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK6AvailabilityAcademic Institutions and Nonprofits only -
pJK_pro6_eGFP
Plasmid#59851PurposeConstitutively expresses eGFP with pro6 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterpro6AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK3_eGFP
Plasmid#59852PurposeConstitutively expresses eGFP with proK3 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK3AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK1_eGFP
Plasmid#59853PurposeConstitutively expresses eGFP with proK1 promoterDepositorInsertEnhanced Green Fluorescent Protein
UseTags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK1AvailabilityAcademic Institutions and Nonprofits only -
pSAG1::CAS9-U6::sg290860-6
Plasmid#59855PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.DepositorInsertCRISPR sg290860-6
UseCRISPR; ; toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
sgFFL
Plasmid#59877Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.DepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_d3
Plasmid#59891Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.DepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--3rd seed site removedPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_d23
Plasmid#59892Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutatedDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--3rd and fourth seed site removedPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_3x_siRNA
Plasmid#59893Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sitesDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--has three siRNA-like targets (fully c…Promoterdoxycycline (Dox)-inducible promoter (CMV/TO)AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_7x_Bulge
Plasmid#59894Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sitesDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--has seven bulge targets (fully comple…Promoterdoxycycline (Dox)-inducible promoter (CMV/TO)AvailabilityAcademic Institutions and Nonprofits only -
OpenLoopControl
Plasmid#59895Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTRDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPESTExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)AvailabilityAcademic Institutions and Nonprofits only -
pMZ374
Plasmid#59896PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1AvailabilityAcademic Institutions and Nonprofits only
Showing: 1181 - 1200 of 144942 results