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Plasmid#159152PurposeCytosolic labile heme reporterDepositorInsertyHS1-M7A,H102A
UseTagsExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterGPDAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K363TAG)-FLAG
Plasmid#182656PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K363 & C-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for amber codon suppression & click labeling of NFL.DepositorInsertmouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK363TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOUPc-UL148-HA
Plasmid#179343PurposeAll-in-one "tet-on" 3G lentiviral vector plasmid encoding human cytomegalovirus UL148 ER resident glycoprotein with a C-terminal HA tag fused to its cytoplasmic tailDepositorInserthuman cytomegalovirus UL148 fused to HA epitope
UseLentiviral and Synthetic Biology; Hiv-1 based rep…TagsHA tagExpressionMammalianMutationinsert matches UL148 from strain TB40/EPromoterTet responsive element 3GAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode
Plasmid#170217PurposeBarcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCASP8 (CASP8 Human)
UseLentiviralTagsV5ExpressionMutationC360APromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Egr3-EYFP-WPRE
Plasmid#186417PurposeOverexpression of Egr3 transcript variant 1 CDS under EF-1α promoterDepositorInsertEarly growth response 3 (Egr3 Mouse)
UseAAVTagsEYFPExpressionMutationPromoterEF-1-alphaAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-218_HNF4A2
Plasmid#31090DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsmycExpressionMammalianMutationsplice variant 2 of HNF4A, deletion of CMV promo…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-138_HNF4A8
Plasmid#31093DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsmycExpressionMammalianMutationsplice variant 8 of HNF4A, deletion of CMV promo…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3-FLIP pr4
Plasmid#19129DepositorInsertFLIP promoter (CFLAR Human)
UseLuciferaseTagsExpressionMammalianMutationThe sequence corresponds to positions -503 to +10…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-68_HNF4A2
Plasmid#31092DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsmycExpressionMammalianMutationsplice variant 2 of HNF4A, deletion of CMV promo…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS
Plasmid#59388PurposeDestination vector with RBPMS for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-138_HNF4A2
Plasmid#31091DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsmycExpressionMammalianMutationsplice variant 2 of HNF4A, deletion of CMV promo…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC19-F4GDP9
Plasmid#103077PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertF4GDP9(Cas9 coding gene from Alicycliphilus denitrificans (strain DSM 14773 / CIP 107495 / K601))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
P2_DD-HNF4A8
Plasmid#31095DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsFKBP L106P and mycExpressionMammalianMutationsplice variant 8 derived from promoter P2PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-gRNA
Plasmid#215547PurposegRNA targeting B2M to introduce splice donor mutation on the first intron of the locusDepositorInsertB2M (B2M Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_FLAG-NFL(K363TAG)
Plasmid#182661PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K363 & an N-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for genetic code expansion & click labeling of NFL.DepositorInsertmouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK363TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 Del EC2
Plasmid#184025PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 273-287.DepositorInsertProm1 SV8(-Ex19a) Del EC2 (Prom1 Mouse)
UseTagsFlagExpressionMammalianMutationDeletion of exon 19a (amino acids 696-701), and d…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZ186
Plasmid#163641Purposerps-27>DHB::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome IDepositorInsertDHB:2xmKate2::3xHA
UseCRISPRTags2xmKate2ExpressionWormMutationPromoterrps-27Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA6
Plasmid#171004PurposeHiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI, R537A, R538A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
prCOrev
Plasmid#153961PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only