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Plasmid#164030PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorInsertCARD8 (CARD8 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationA452C, Y527APromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2560 - pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Plasmid#159825PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertpEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
Plasmid#159817PurposePositive control for germline GFP expression from simple array in C. elegansDepositorInsertpEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Casp8-mod
Plasmid#48171Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> ATT) upstream open reading frame elementDepositorInsert5" UTR of CASP8 (CASP8 Human)
UseLuciferaseTagsExpressionMutationG/T MUTATION IN ATG OF UORFPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
Twinkle-APEX2-V5
Plasmid#129705PurposeAPEX2 fused to the mitochondrial nucleoid protein TwinkleDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MYR-FR-C2D-EGFR
Plasmid#179262PurposeLight-induced clustering of EGFR cytosolic domainsDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsCry2 PHR, FUS, FusionRed, and Myristoylation sequ…ExpressionMutationPromoterSFFVAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1
Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
Plasmid#221081PurposeSEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-N-FLAG-HA-TBK1
Plasmid#131791PurposeLentiviral vector expressing WT TBK1DepositorInsertTBK1 WT (TBK1 Human)
UseLentiviralTagsN-FLAG-HAExpressionMammalianMutationWT full lengthPromoterCMVAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pC-BAR-YR
Plasmid#61766PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).DepositorInsertsBar gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromotertrpC promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-XBP1 mNeonGreen NLS
Plasmid#115971PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)DepositorInsertX-box binding protein 1 (XBP1 Synthetic, Human)
UseRetroviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
APEX2 - Actin in pEGFP
Plasmid#66172PurposeAPEX2 fused to the N-terminus of human beta actinDepositorInsertAPEX2 - human beta Actin (ACTB Synthetic, Human)
UseTagsFlag epitope tag (DYKDDDDK)ExpressionMammalianMutationAPEX2 contains 4 mutations relative to wt soybean…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pADC10-SETXhel
Plasmid#193056PurposeBaculovirus expression of human Senataxin helicase domain for protein productionDepositorInsertSenataxin (SETX Human)
UseTagsFlagExpressionInsect and MammalianMutationcodon optimized for insect cell expressionPromoterhybrid p10 (insect) and CMV (human) expressionAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-TORCAR
Plasmid#64929PurposeLysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.DepositorInsertLAMP1-TORCAR (LAMP1 Synthetic, Human)
UseTagsCerulean, Full-length LAMP1, and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCOTS-pyl-GFP(35TAG)
Plasmid#92047PurposeThis is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.DepositorInsertsEGFP
Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseReplicative expression plasmid for cyanobacteria …TagsHistagExpressionMutationTyrosine in position 35 of the GFP was mutated f…PromoterLeuP (native S. elongatus promoter), PpsbII, and …Available sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso1E335A
Plasmid#159587PurposeExpress N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationE335APromoterCMVAvailable sinceMay 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYX233-DHFR
Plasmid#163761PurposeGal-inducible yeast expression of cytosolic DHFRDepositorInsertDHFR (Dhfr Mouse)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only