Addgene

We narrowed to 14,887 results for: grna

Showing: 11501 - 11520 of 14887 results

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MTK234_010

Plasmid

#123903

Purpose

Encodes the spCas9 pTRE gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TetO-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_016

Plasmid

#123904

Purpose

Encodes the saCas9 pTRE gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TetO-SasgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_021

Plasmid

#123905

Purpose

Encodes the spCas9 pUAS gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA-UAS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_026

Plasmid

#123906

Purpose

Encodes the spCas9 human non-targeting gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

human non targeting sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_031

Plasmid

#123911

Purpose

Encodes the spCas9 sgRNA1 hCLYBL (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA2 -hCLYBL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_039

Plasmid

#123914

Purpose

Encodes the spCas9 tagBFP gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 tagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_040

Plasmid

#123915

Purpose

Encodes the spCas9 mAzamiGreen gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mAzamiGreen sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL322 GC

Plasmid

#126950

Purpose

To target a protospacer with GC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GC at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL581 GA

Plasmid

#126951

Purpose

To target a protospacer with GA at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GA at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL601 AG

Plasmid

#126952

Purpose

To target a protospacer with AG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AG at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL602 CG

Plasmid

#126953

Purpose

To target a protospacer with CG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CG at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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PCDHGC5 sh1 shRNA

Plasmid

#122227

Purpose

Knock Down Protocadherin gamma C5. It targets Protocadherin gamma C5 mRNA (nucleotides 851–871 in the protein coding region).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 shRNA (Pcdhgc5 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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PCDHGC5 sh1 3m shRNA

Plasmid

#122228

Purpose

Negative control shRNA (with 3-point mutations), for plasmid 122227: targeting Protocadherin gamma C5 mRNA.

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 shRNA (Pcdhgc5 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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gamma2 UTR shRNA (shRNA4)

Plasmid

#120796

Purpose

shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

Depositor

Angel de Blas

Article

Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

Insert

GABRG2 shRNA (Gabrg2 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h1 ts3

Plasmid

#115882

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shLARP7

Plasmid

#113537

Purpose

Retroviral vector designed to knock down LARP7.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shLARP7 (LARP7 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgAMDHD1

Plasmid

#102316

Purpose

genetic depletion of AMDHD1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgAMDHD1 (AMDHD1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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TRIN-E-shTAF12.364

Plasmid

#105572

Purpose

Dox-inducible mir30 MYB shRNA/dsRED expression with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-shScn4b-268

Plasmid

#99031

Purpose

Expresses a shRNA targeting the mouse Scn4b gene

Depositor

Amy Lasek

Article

Ji et al Front Cell Neurosci. 2017 Feb 13;11:17.

Insert

Scn4b shRNA (Scn4b Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pX335_HR_Prnp_3

Plasmid

#78622

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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