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Plasmid#146971PurposeInsect Expression of DmGW182_1-605-dsRNAresDepositorInsertDmGW182_1-605-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182_1-830-dsRNAres_N
Plasmid#146972PurposeInsect Expression of DmGW182_1-830-dsRNAresDepositorInsertDmGW182_1-830-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182_1-1115-dsRNAres_N
Plasmid#146973PurposeInsect Expression of DmGW182_1-1115-dsRNAresDepositorInsertDmGW182_1-1115-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationtwo non silent mutations V704A and N799S, and one…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmXRN1_1312-1383-V5His6_N
Plasmid#146975PurposeInsect Expression of DmXRN1_1312-1383DepositorInsertDmXRN1_1312-1383 (pcm Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmXRN1_1323-1355-V5His6_N
Plasmid#146976PurposeInsect Expression of DmXRN1_1323-1355DepositorInsertDmXRN1_1323-1355 (pcm Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delM2-dsRNAres_H
Plasmid#146438PurposeInsect Expression of DmGW182-delM2-dsRNAresDepositorInsertDmGW182-delM2-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGe1_1-943-V5His6_G
Plasmid#146350PurposeInsect Expression of DmGe1_1-943DepositorInsertDmGe1_1-943 (Ge-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGe1_944-1354-V5His6_G
Plasmid#146351PurposeInsect Expression of DmGe1_944-1354DepositorInsertDmGe1_944-1354 (Ge-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmUPF3-siRNAres-V5His6_E
Plasmid#146167PurposeInsect Expression of DmUPF3-siRNAresDepositorInsertDmUPF3-siRNAres (Upf3 Fly)
UseTagsExpressionInsectMutation5 silent and one non silent mutation P305S compar…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-tdTomato-sgRNA
Plasmid#194724Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target tdTomatoDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mGFP-hNET-R121A/K334A/R440A
Plasmid#193366Purposeexpression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cellsDepositorInserthuman Norepinephrine Transporter (SLC6A2 Human)
UseTagsmonomeric GFP (mGFP)ExpressionMammalianMutationR121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …PromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-β-catenin_S23A,T40A,T41A,T112A
Plasmid#190824PurposeFor mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.DepositorInsertβ-catenin (CTNNB1 Chicken)
UseTagsGFPExpressionMammalianMutationS23A, T40A, T41A, T112APromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-del72-136-pEGFP-C3
Plasmid#191411PurposeTransfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).DepositorInsertBcl-2-interacting killer (BIK Human)
UseTagsVenusExpressionMammalianMutationwith deletion of amino acids 72-136 (del72-136) i…PromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3A tail 419-702
Plasmid#193923PurposeMammalian expression of GFP-tagged Kif 3A tail 419-702DepositorInsertKif 3A (KIF3A Human)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-p15 Bid-KKAA-GFP
Plasmid#191970PurposeExpression of the active p15 cBid KKAA mutant, chimera with GFP.DepositorInsertp15 - cBid KKAA
UseTagsExpressionMammalianMutationKKAA: Lys 157 and Lys 158 into AlaPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-GFP::NMN-Deamidase{dead}
Plasmid#187869PurposeGal4/UAS expression of GFP::NMN-Deamidase(dead)DepositorInsertNMN-Deamidase{dead}
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-EGFP
Plasmid#191458PurposeCompare the intensity of EGFP to mScarlet-I fluorescent proteinDepositorInsertEGFP
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOSR1-EGFP-PGK-Puro
Plasmid#186170PurposeKnock-in gene targeting in marmoset cells at OSR1 locusDepositorInsertEGFP
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax
Plasmid#190154PurposeExpresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertVamp3 (Vamp2 Rat)
UseAAVTagsP2AT2A-EGFP-caaxExpressionMammalianMutationTruncation that includes only rat Vamp3 AA #1-81PromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV105-PPAB1-GFP-Erk1R84S-1SIM
Plasmid#190253PurposeExpression Erk1-GFP-1SIM in yeast cellsDepositorInsertERK1R84S-SIM
UseTagsGFPExpressionYeastMutationERK1R84SPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only