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Condensed-phase signaling can expand kinase specificity and respond to macromolecular crowding.
Sang D, Shu T, Pantoja CF, Ibanez de Opakua A, Zweckstetter M, Holt LJ
Mol Cell. 2022 Oct 6;82(19):3693-3711.e10. doi: 10.1016/j.molcel.2022.08.016. Epub 2022 Sep 14. PubMed Article

Plasmids from Article

ID Plasmid Purpose
190248pRS306-PTDH3-SUMO10-6SIMExpression SUMO10-SIM6 in yeast cells
190249pRS306-PTDH3-Dimer-1SUMOExpression dimer-1SUMO component in yeast cells
190250pRS306-PTDH3-SUMO7-6SIMExpression SUMO7-SIM6 in yeast cells
190251pRS304-PTDH3-HEXAMERExpression hexamer component in yeast
190252pAV103-PRNR2-mCherry-Elk1DM-1SIMExpression mCherry-Elk1DM in yeast cells
190253pAV105-PPAB1-GFP-Erk1R84S-1SIMExpression Erk1-GFP-1SIM in yeast cells

Antibodies from Article