The NAD(+) precursor NMN activates dSarm to trigger axon degeneration in Drosophila.
Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, Wu PH, Coleman MP, Loreto A, Neukomm LJ
Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245. PubMed Article
Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, Wu PH, Coleman MP, Loreto A, Neukomm LJ
Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187868 | UAS-GFP::NMN-Deamidase | Gal4/UAS expression of GFP::NMN-Deamidase |
| 187869 | UAS-GFP::NMN-Deamidase{dead} | Gal4/UAS expression of GFP::NMN-Deamidase(dead) |
| 187870 | UAS-dsarm(E) | Gal4/UAS expression of dSarm(isoE) |
| 187871 | UAS-dsarm(E)::flag | Gal4/UAS expression of dSarm(isoE)::FLAG |
| 187881 | UAS-dsarm(E,K450R) | Gal4/UAS expression of dSarm(isoE,K450R) |
| 187882 | UAS-dsarm(E,K450R)::flag | Gal4/UAS expression of dSarm(isoE,K450R)::FLAG |
| 187883 | UAS-4x(tRNA::axed{sgRNA}) | Gal4/UAS sgRNA expression targeting axed |
| 187884 | UAS-4x(tRNA::hiw{sgRNA}) | Gal4/UAS sgRNA expression targeting hiw |
| 187885 | UAS-4x(tRNA::dsarm{sgRNA}) | Gal4/UAS sgRNA expression targeting dsarm |
| 187887 | UAS-mNAMPT | Gal4/UAS expression of mammalian NAMPT |