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Showing: 1101 - 1120 of 14475 results

  4. pOCC230

    Plasmid
    #118871
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and mCitrine, cl…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118871-plasmid-map-sequence-id-232332

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  5. pOCC69

    Plasmid
    #118874
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and mGFP, cleava…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118874-plasmid-map-sequence-id-232330

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  11. pCS2+-Zeo-miR-430-G-G mm

    Plasmid
    #221728
    Purpose
    Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.
    Depositor
    David Bartel
    Article
    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5
    Insert
    BleoR-miR-430-G-G mm
    Use
    Fish expression
    Tags
    Expression
    Mutation
    G-G mismatch of miR-430 site
    Promoter
    simian CMV IE94
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221728-plasmid-map-sequence-id-449658

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  12. pCS2+-Zeo-miR-430-10-11 mm

    Plasmid
    #221727
    Purpose
    Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.
    Depositor
    David Bartel
    Article
    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5
    Insert
    BleoR-miR-430-10-11 mm
    Use
    Fish expression
    Tags
    Expression
    Mutation
    Mismatch at positions 10 and 11 of miR-430 site
    Promoter
    simian CMV IE94
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221727-plasmid-map-sequence-id-449656

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  15. KBB430

    Plasmid
    #185091
    Purpose
    NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)
    Depositor
    Ken Belanger
    Article
    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4
    Insert
    NMA111
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    First nuclear localization signal mutated (amino …
    Promoter
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185091-plasmid-map-sequence-id-368167

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  16. KBB428

    Plasmid
    #185090
    Purpose
    NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)
    Depositor
    Ken Belanger
    Article
    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4
    Insert
    NMA111
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    First nuclear localization signal mutated (amino …
    Promoter
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185090-plasmid-map-sequence-id-367998

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  20. Banshee-ShCit-A

    Plasmid
    #85216
    Purpose
    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.
    Depositor
    Alex Minella
    Article
    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002
    Insert
    shCit-A
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1
    Available since
    Feb. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    85216-plasmid-map-sequence-id-171277

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Showing: 1101 - 1120 of 14475 results