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Showing: 2751 - 2800 of 14285 results

  2. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

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  9. pSAM_Cam

    Plasmid
    #91570
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. pWJ66

    Plasmid
    #46570
    Purpose
    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9-w
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46570-plasmid-map-sequence-id-65846

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  23. pIVM_02

    Plasmid
    #204500
    Purpose
    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)
    Depositor
    Alessio Ciulli
    Article
    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.
    Insert
    amino acids 54-213 VHL (VHL Human)
    Use
    Tags
    6xHis, TEV, and Thrombin
    Expression
    Bacterial
    Mutation
    0
    Promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204500-plasmid-map-sequence-id-406419

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  25. BiP-mGFP

    Plasmid
    #62231
    Purpose
    Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.
    Depositor
    Erik Snapp
    Article
    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2
    Insert
    hamster BiP-mGFP-KDEL
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62231-plasmid-map-sequence-id-314151

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  47. p2R3z-BASI-ccdB-BSAI

    Plasmid
    #118389
    Purpose
    Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4
    Insert
    ccdB
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    118389-plasmid-map-sequence-id-237126

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  50. pKO (Apr)

    Plasmid
    #110088
    Purpose
    Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Bacterial knockout generation
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110088-plasmid-map-sequence-id-211180

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Showing: 2751 - 2800 of 14285 results