GFP-PKCA WT
(Plasmid
#195202)
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PurposeMammalian expression vector for GFP tagged PKCA.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195202 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-PKCA WT was a gift from David Bryant & Peter Parker (Addgene plasmid # 195202 ; http://n2t.net/addgene:195202 ; RRID:Addgene_195202) -
For your References section:
PKC maturation is promoted by nucleotide pocket occupation independently of intrinsic kinase activity. Cameron AJ, Escribano C, Saurin AT, Kostelecky B, Parker PJ. Nat Struct Mol Biol. 2009 Jun;16(6):624-30. doi: 10.1038/nsmb.1606. Epub 2009 May 24. 10.1038/nsmb.1606 PubMed 19465915