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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Promoter

Availability

Academic Institutions and Nonprofits only

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pJCC_076 AsCas12a R1226A

Plasmid

#171669

Purpose

For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a R1226A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginine 1226 to alanine

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

Plasmid

#175525

Purpose

Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag10 and Tomm20-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

Promoter

Availability

Academic Institutions and Nonprofits only

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pCW57-GFP-2A-Cas9

Plasmid

#170805

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

Depositor

Morten Frodin

Article

Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP5H

Plasmid

#188061

Purpose

PROSS-designed versatile peroxidase 5H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Use

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

43 PROSS mutations, described in publication

Promoter

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP8H

Plasmid

#188062

Purpose

PROSS-designed versatile peroxidase 8H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1

Use

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

38 PROSS mutations, described in publication

Promoter

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP11H

Plasmid

#188063

Purpose

PROSS-designed versatile peroxidase 11H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Use

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

38 PROSS mutations, described in publication

Promoter

Availability

Academic Institutions and Nonprofits only

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pDR111_Bgal NLS

Plasmid

#188396

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Use

Tags

PhoD secretion peptide and SV40 NLS

Expression

Bacterial

Mutation

Promoter

Phyper spank

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Bgal NLS

Plasmid

#188398

Purpose

Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Use

Tags

PhoD secretion peptide and SV40 NLS

Expression

Bacterial

Mutation

Promoter

D-mannose

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e KRAB

Plasmid

#188506

Purpose

Expresses FLAG tagged PlmdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-KRAB-NLS

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_3XNLS-MicroID2-FLAG

Plasmid

#188523

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

3XNLS-MicroID2

Use

Transposon mediated gene integration

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_MicroID2-FLAG-NES

Plasmid

#188525

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-K14R

Plasmid

#185759

Purpose

Bacterial expression of His6-Ubc9-K14R

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Insert

SUMO-conjugating enzyme UBC9

Use

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

K14R

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28b_RanT24N

Plasmid

#186271

Purpose

Expression vector of His-RanT24N in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran

Use

Tags

His tagged and His-tagged at N terminal

Expression

Bacterial

Mutation

Changed T24 into N

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pET28b_Q69L

Plasmid

#186272

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran

Use

Tags

His-tagged at N terminal

Expression

Bacterial

Mutation

Changed Q69 into L

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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ArpC3-FLAG_pLib

Plasmid

#173680

Purpose

ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC3

Use

Tags

TEV cleavage site and FLAG tag

Expression

Insect

Mutation

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

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pPIK16045_pX330_Flag-SuperFi-Cas9

Plasmid

#184370

Purpose

Mammalian expression of SuperFi-Cas9 nuclease

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG and NLS

Expression

Mammalian

Mutation

Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

Promoter

CBh

Availability

Academic Institutions and Nonprofits only

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pJH4542

Plasmid

#200779

Purpose

Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GCaMP6

Use

Tags

mNeptune

Expression

Worm

Mutation

Promoter

Ptwk-40

Availability

Academic Institutions and Nonprofits only

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pYG205

Plasmid

#200836

Purpose

Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives

Depositor

Boris Görke

Article

Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

Insert

rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P-BAD

Availability

Academic Institutions and Nonprofits only

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