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pCFD4d-U6-1:white1-U6-3:white1

Plasmid

#84005

Purpose

pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

Depositor

Phillip Zamore

Article

Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

Inserts

white sgRNA-1
white sgRNA-1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6:1, U6:3, and dU6-1; dU6-3

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MORC2 ts2

Plasmid

#115887

Purpose

MORC2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MORC2 shRNA (MORC2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-Tet1

Plasmid

#85742

Purpose

shRNA against mouse Tet1

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA Tet1

Use

AAV

Tags

EYFP

Expression

Mutation

Promoter

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-dsRed-FF3

Plasmid

#11664

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of dsRed and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

dsRed

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only
pXD017-39 TRAC PDCD1 AAV double KO AAV

Plasmid

#192153

Purpose

TRAC PDCD1 AAV double KO AAV

Depositor

Sidi Chen

Article

Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

Insert

sgRNA

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-pX330-Cas9-mCherry

Plasmid

#159742

Purpose

Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

donor plasmid targeting sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPbU6_hdhfr/yfcu_Cas9

Plasmid

#216423

Purpose

Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

Depositor

Ellen Bushell

Article

Jonsdottir et al bioRxiv 2024.04.20.590404

Type

Empty backbone

Use

CRISPR; Expression in plasmodium berghei

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2024

Availability

Academic Institutions and Nonprofits only

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sgMLST8_1

Plasmid

#124905

Purpose

Targeting human MLST8 gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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px459 sgAtg5

Plasmid

#175023

Purpose

CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

Depositor

David Tumbarello

Article

Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

Insert

ATG5 (ATG5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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PLKO.1-CAPRIN1-3'UTR

Plasmid

#136055

Purpose

CAPRIN1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCACGTTAGTGTCACAAATT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

CAPRIN1 (CAPRIN1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_IDH2

Plasmid

#183301

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FLT4

Plasmid

#183294

Purpose

All-in-One CRISPRko system with a guide RNA that targets FLT4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FLT4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

Plasmid

#191397

Purpose

sgRNA targeting alpha-satellites on human chromosome 9

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

sgRNA targeting alpha satellites on human chromosome 9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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Luc-pSUPER-Retro-GFP/Neo

Plasmid

#22133

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-GFP Smad2

Plasmid

#15722

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad2 shRNA (SMAD2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pSicoR-ADAM29i1

Plasmid

#59606

Purpose

Expression of shRNA against human ADAM29

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM29 (ADAM29 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pVG1

Plasmid

#111444

Purpose

Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2/stop-codon repair

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LEP-shREN

Plasmid

#105580

Purpose

retrovirally express control shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-shSrc

Plasmid

#26982

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Src (SRC Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_Luc-A

Plasmid

#25760

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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