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Plasmid#58317PurposeFLAG-tagged mouse TRAF4 K190R mutantDepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsFLAGExpressionMammalianMutationK 190 to RPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 LNG (CC→SS)-6MT
Plasmid#41739DepositorInsertNotch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
UseTags6xMycExpressionMammalianMutationContains murine Notch-1 leader peptide (aa 1-23) …PromoterAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
5mARF17 pGreenII0229
Plasmid#12071DepositorInsert5mARF17 (ARF17 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionPlantMutationmiR160-resistant version of ARF17 has five silent…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEB5 (mANGPTL8-V5-His lenti)
Plasmid#109115PurposeV5 and His-tagged mouse ANGPTL8 in a lentiviral vectorDepositorInsertANGPTL8 (Angptl8 Mouse)
UseLentiviralTags6x His and V5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral FL
Plasmid#113012PurposeFor bacterial expression of MBP fusion of full-length Drosophila Tral WTDepositorInsertfull length tral (tral Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-577-CFP-578CT
Plasmid#41682DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; CFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-577-YFP-578CT
Plasmid#41681DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; YFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-C12AC15A-LIN-41
Plasmid#52725Purposeretroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationchanged cysteines 12 and 15 to alaninesPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1CFP*
Plasmid#41666DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; C-terminal hyd…PromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
BMR1_03g04550-bio-His
Plasmid#108026PurposeExpresses enzymatically monobiotinylated full-length BMR1_03g04550 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertBMR1_03g04550
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
BMR1_02g00615-bio-His
Plasmid#108029PurposeExpresses enzymatically monobiotinylated full-length BMR1_02g00615 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertBMR1_02g00615
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Hes3
Plasmid#61535PurposeExpresses Hes3 in mammalian cellsDepositorInsertHes3 (Hes3 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_nmPKA-Cα-mPAGFP
Plasmid#114131PurposeMammalian expression of the G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertof the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with G2A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
5mARF17 pGreenII0129
Plasmid#12081DepositorInsert5mARF17 (ARF17 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionPlantMutationmiR160-resistant version of ARF17 has five silent…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K651R
Plasmid#84536PurposeThis plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK651RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Myc-TRFcM
Plasmid#44575DepositorUseRetroviralTagsMycExpressionMammalianMutationTRF2 DNA-binding domain (aa 444-500) is replaced …PromoterAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRLCon850-1207
Plasmid#31447DepositorInsertHNF4A 3'UTR fragment (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pTSlb-R756K
Plasmid#60731PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP
Plasmid#89062PurposeAAV-gRNA targeting the murine Apob geneDepositorInsertEmGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterChicken beta actin with partial CMV enhancerAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only