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Plasmid#118330PurposeExpression of human BrafDepositorInsertBraf R188L (BRAF Human)
UseTagsGFPExpressionMammalianMutationR188LPromoterCMVAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
SNAP-CLC-EGFP
Plasmid#193579PurposeExpresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsSNAP-tag and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
A*68:01:01
Plasmid#203292PurposeOverexpress HLA alleleDepositorInsertA*68:01:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
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7432 Bicistronic_GFP_ires_puro
Plasmid#64336PurposeThis a retroviral expression plasmid expressing GFP along with puro resistance geneDepositorInsertGFP
UseRetroviralTagsGFPExpressionMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAHS2parrc-mCh-2xFKBP (pBS1109)
Plasmid#185304PurposeFor the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertCAHS2parrc
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1 pET151
Plasmid#159384PurposeExpresses human TLNRD1 in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 (TLNRD1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
G-GECO1.2-Orai1Y80E
Plasmid#73565PurposeGreen fluorescent reporter of mutant Orai1Y80E-associated calcium influxDepositorInsertG-GECO1.2-Orai1Y80E (ORAI1 Human, Synthetic)
UseTagsG-GECO1.2ExpressionMammalianMutationchanged tyrosine 80 of Orai1 to glutamatePromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-1Cre
Plasmid#98038PurposeSecond-generation rabies viral vector genomeDepositorInsertmCre
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mNeonGreen-CI
Plasmid#196850PurposeExpression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoterDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-1: shALAS-1
Plasmid#22750DepositorInsertshALAS-1 (Alas1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
3611 pcDNA3 Bim
Plasmid#8804DepositorInsertBim (Bcl2l11 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-deltaRING-LIN-41
Plasmid#52718Purposeretroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 12-91PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-Q758C
Plasmid#60729PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758CDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSNR52-sgTET
Plasmid#46923PurposeYeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoterDepositorInsertsgRNA targeting endogenous TRE elements of pTET07 promoter
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDIV494
Plasmid#177701PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ), SNR52p (Candida s…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4EGFP
Plasmid#98037PurposeSecond-generation rabies viral vector genomeDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Tet1 promoter A
Plasmid#63881PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet1DepositorInsertTet1 promoter fragment A
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pF711-pET28a-Hs-delta25-MCAD-NHis wt
Plasmid#85112Purposeexpression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta25) MCAD (ACADM Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
RCASBP(A)-3xFlag-mHoxd13wt
Plasmid#145288PurposeAvian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag tagDepositorInsertHoxd13(wt) (Hoxd13 Mouse)
UseViral avian expressionTags3xFlagExpressionMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only