GFP-Braf R188L
(Plasmid
#118330)
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PurposeExpression of human Braf
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerinvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBraf R188L
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SpeciesH. sapiens (human)
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Insert Size (bp)2301
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MutationR188L
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Entrez GeneBRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
site directed mutagenesis. BRaf constructs were subcloned into pcDNA3 containing GFP epitope with NotI and XbaI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-Braf R188L was a gift from Philip Stork (Addgene plasmid # 118330 ; http://n2t.net/addgene:118330 ; RRID:Addgene_118330) -
For your References section:
Ras-mutant cancer cells display B-Raf binding to Ras that activates extracellular signal-regulated kinase and is inhibited by protein kinase A phosphorylation. Li Y, Takahashi M, Stork PJ. J Biol Chem. 2013 Sep 20;288(38):27646-57. doi: 10.1074/jbc.M113.463067. Epub 2013 Jul 26. 10.1074/jbc.M113.463067 PubMed 23893412