Addgene

We narrowed to 20,487 results for: crispr

Showing: 10441 - 10460 of 20487 results

Results per page:

Sort results by:

2X_pX458_pSpCas9(BB)-2A-GFP

Plasmid

#172221

Purpose

Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pgRNAtet-lvaA

Plasmid

#106397

Purpose

Guide RNA plasmid targeting lvaA on BBR1-UP origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

sgRNA towards lvaA in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ALKBH5H204A-dCas9

Plasmid

#134784

Purpose

Expression fusion protein ALKBH5H204A-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

ALKBH5 and inactive dCas9

Use

Tags

Expression

Mammalian

Mutation

H204A

Promoter

CMV

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
eSpCas9(1.1)

Plasmid

#71814

Purpose

Expresses high specificity SpCas9. Px330-like plasmid.

Depositor

Feng Zhang

Article

Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

Has Service

Cloning Grade DNA

Insert

enhanced specificity Cas9 (1.1)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TO-Sp dCas9-mVenusN

Plasmid

#191396

Purpose

SpdCas9 fused to N-terminal part of mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

N-terminal part of split mVenus

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_BE4max

Plasmid

#112093

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hCas9_D10A

Plasmid

#41816

Purpose

Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9_D10A

Use

CRISPR

Tags

Expression

Mammalian

Mutation

human codon-optimized, D10A nickase

Promoter

CMV

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_BE4max_3xHA

Plasmid

#112096

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-PspCas13b-gRNA-Actb1216

Plasmid

#155368

Purpose

PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

PspCas13b gRNA targeting Actb 1216

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFD5-frame_selector_0,1,2

Plasmid

#131152

Purpose

Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

CRISPaint frame selector sgRNAs 0,1,2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6:3

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-AncBE4max

Plasmid

#138270

Purpose

Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter.

Depositor

Xiao Wang

Article

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

AncBE4max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A

Promoter

Ef1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

Plasmid

#128424

Purpose

Human Codon Optimized dCas9-DNMT3A3L

Depositor

Bradley Bernstein

Article

Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

Insert

dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

T7 Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE TRE dCas9

Plasmid

#50915

Purpose

Tet-regulatable dCas9 lentiviral expression vector

Depositor

Scot Wolfe, Rene Maehr

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pN7HELIX

Plasmid

#190662

Purpose

Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB

Use

Tags

Expression

Mammalian

Mutation

nAniI = K227M, F80K, L232K

Promoter

CMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PEmax-mRNA

Plasmid

#204472

Purpose

plasmid for PEmax mRNA IVT

Depositor

Scot Wolfe

Article

Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

Insert

Prime editor

Use

CRISPR

Tags

no

Expression

Mammalian

Mutation

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FTO-dCas9

Plasmid

#134781

Purpose

Expression fusion protein FTO-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

FTO and inactive dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6a:sgRNA(tyr)

Plasmid

#64250

Purpose

expresses sgRNA(tyr) under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

U6a:sgRNA (tyr)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pScI_dCas9-CDA_J23119-sgRNA

Plasmid

#108550

Purpose

Bacterial Target-AID vector with sgRNA

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Inserts

dCas9-PmCDA1
Streptococcus pyogenes sgRNA

Use

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

J23119 and lambda OR

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
2xNLS-pMJ915v2

Plasmid

#88916

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge