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PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41816 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.3-TOPO
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCas9_D10A
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Insert Size (bp)4140
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Mutationhuman codon-optimized, D10A nickase
- Promoter CMV
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV_F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For additional information:
http://arep.med.harvard.edu/human_crispr/
For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hCas9_D10A was a gift from George Church (Addgene plasmid # 41816 ; http://n2t.net/addgene:41816 ; RRID:Addgene_41816) -
For your References section:
RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033 10.1126/science.1232033 PubMed 23287722