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Showing: 2551 - 2600 of 156554 results

  3. mCherry-Keratin-17

    Plasmid
    #55065
    Purpose
    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Keratin (KRT18 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    V282L
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60621-plasmid-map-sequence-id-208224

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  10. pcDNA3.1(+)-HLA-Flag-natT1R3

    Plasmid
    #113950
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from HLA class I …
    Expression
    Mammalian
    Mutation
    residues 21-852
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113950-plasmid-map-sequence-id-224188

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  15. TFORF0707

    Plasmid
    #143889
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOR1 (NCOR1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    143889-plasmid-map-sequence-id-408610

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  18. optoAKT

    Plasmid
    #207164
    Purpose
    Light-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathway
    Depositor
    Kai Zhang
    Article
    Wang et al Elife. 2020 Oct 6;9:e57395. doi: 10.7554
    Insert
    CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    207164-plasmid-map-sequence-id-413072

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  24. mCherry-ZO1-C-14

    Plasmid
    #55165
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55165-plasmid-map-sequence-id-194785

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  25. B-GRAM-W

    Plasmid
    #211707
    Purpose
    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211707-plasmid-map-sequence-id-419219

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  26. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212963
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_GFP targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212963-plasmid-map-sequence-id-422499

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  36. pAB1

    Plasmid
    #62547
    Purpose
    broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.
    Depositor
    Michael Franklin
    Article
    Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47
    Insert
    eGFP
    Use
    Broad host range plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTrc
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    62547-plasmid-map-sequence-id-314155

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  41. mCherry-Rab11a-7

    Plasmid
    #55124
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab11a (RAB11A Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55124-plasmid-map-sequence-id-127231

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  43. pDEST17-His10-ccdB

    Plasmid
    #166145
    Purpose
    Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.
    Depositor
    Daniel Jarosz
    Article
    Itamar Harel et al BioRxiv
    Type
    Empty backbone
    Use
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    166145-plasmid-map-sequence-id-328877

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Showing: 2551 - 2600 of 156554 results