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pB-puro-mU6-CNPshRNA1

Plasmid

#126611

Purpose

Expresses shRNA against human CNP under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP ShRNA-1 (CNP Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-eGFP-IRESMuro

Plasmid

#82504

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP-IRESMuro

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-WDR5 shRNA 4

Plasmid

#59977

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only
pSR-puro-RalBP1

Plasmid

#31115

Depositor

Christopher Counter

Article

Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

Insert

RalBP1 shRNA (RALBP1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
pIRESpuro3+mRFP-Utr_CH

Plasmid

#64358

Purpose

bicistronic expression of Utrophin-mRFP and Puro resistance

Depositor

Matthieu Piel

Insert

Utrophin-mRFP (UTRN Human)

Use

Tags

IRES-Puro and mRFP

Expression

Mammalian

Mutation

Q236R; aa1-261 Utrophin present

Promoter

CMV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSR-puro-Sec5

Plasmid

#31117

Depositor

Christopher Counter

Article

Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

Insert

Sec5 shRNA (EXOC2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
pB-puro-mU6-CNPsgRNA

Plasmid

#126610

Purpose

Expresses CNP sgRNA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP sgRNA (Cnp Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE.puro/TO/Flag/tGFP.HuR.S221D

Plasmid

#110351

Purpose

Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and turboGFP tags

Depositor

Sandra Martha Gomes Dias

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Retroviral

Tags

FLAG and tGFP

Expression

Mammalian

Mutation

S221D, P237S

Promoter

gag

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-PuroTK-L2

Plasmid

#141005

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

PuroTK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-PKN1 shRNA 4

Plasmid

#59978

Purpose

Knockdown of PKN1

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

PKN1 shRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfPar3_4

Plasmid

#26706

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

Dog Par3 RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Dog Par3 RNAi

Promoter

Available since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only
PSuperPuroRetro-1660s(hPot1)

Plasmid

#11136

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against Human Protection of Telomeres 1 (control) (POT1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pFIV-H1-Puro-hPolß

Plasmid

#18663

Depositor

Robert Sobol

Article

Trivedi et al Molecular Pharmacology 2008;74(2):505-16

Insert

shRNA specific to human DNA polymerase beta (POLB Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Oct. 24, 2008

Availability

Academic Institutions and Nonprofits only
pLG1-puro-sgATL3-2

Plasmid

#109010

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #2 (ATL3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-1

Plasmid

#109008

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #1 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE.puro/TO/Flag/mKO2

Plasmid

#110353

Purpose

Doxycycline inducible mammalian retroviral expression of mKO2 with FLAG tag

Depositor

Sandra Martha Gomes Dias

Insert

monomeric Kusabira Orange 2

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

gag

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shPIK3CD.v1 puro

Plasmid

#58706

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
pOZ-Pax7-FH-C-puro

Plasmid

#32518

Depositor

Andrew Lassar

Article

Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. E

Insert

Pax7d (Pax7 Mouse)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pBABEpuro-HA-MEK1 CBM

Plasmid

#53196

Purpose

Expresses HA tagged human MEK1 M187A/H188A/M230A/H239A from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

M187A/H188A/M230A/H239A

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRab8a_2

Plasmid

#26707

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rab8a RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Dog Rab8a RNAi.

Promoter

Available since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only