GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives.
Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E, Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ, Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 10.1016/j.stemcr.2016.07.015.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
82334 | pGAPTrap-Clover-Muro | Vector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene. |
82335 | pGAPTrap-mtagBFP2-IRESMuro | Vector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene. |
82336 | pGAPTrap-IRESMuro | Base vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene. |
82355 | pGAPTrap-mtagtdTom_IRESMuro | Vector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene. |
82502 | pGAPTrap-eGFP-IRESMeo | Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene. |
82503 | pGAPTrap-eGFP-IRESMygro | Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene. |
82504 | pGAPTrap-eGFP-IRESMuro | Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene. |
82505 | pGAPTrap-mCherry-IRESMygro | Vector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene. |
82506 | pGAPTrap-GFP | Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. |
82507 | pGAPTrap-LacZ-IRESMuro | Vector targeting the LacZ gene to the GAPDH locus of human cells. LacZ is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene. |
82509 | pGAPTrap-Luc2-IRESMeo | Vector targeting the Firefly Luciferase (Luc2) gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Meo gene. |
83368 | pGAPTrap-TALEN1 | Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors |
83369 | pGAPTrap TALEN 2 | Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors |