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Plasmid#183936PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VPR
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc
Plasmid#25458DepositorInsertPAM (Pam Rat)
UseTagsHis6 and PAM signalExpressionMammalianMutationContains aa 498-820 (PALcc= PAL catalytic core) …PromoterAvailable sinceJuly 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-HA-NLS-GFP4-VPR
Plasmid#183924PurposeVPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacerDepositorInsertdCas9-GFP4-VPR
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-TetR-NLS-VP16
Plasmid#103835PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arraysDepositorInserttagBFP-TetR-NLS-VP16 (tetR )
UseTagsExpressionMammalianMutationPromoterSV2Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-VHL-Flag(117-213)
Plasmid#29501DepositorInsertVon Hippel-Lindau (VHL Human)
UseTagsFlag and HisExpressionInsectMutationVHL(117-213) onlyPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-p58
Plasmid#166942Purposemammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFPDepositorInsertp58 (Lman1 Rat)
UseTagsGFP and chicken lysozyme signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec61b-C1
Plasmid#90994PurposeExpresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cellsDepositorInsertmCherry-Sec61b (SEC61B Human, Synthetic)
UseTagsmCherry (red fluorescence)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Sec61b-C1
Plasmid#90992PurposeExpresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cellsDepositorInsertmEmerald-Sec61b (SEC61B Human, Synthetic)
UseTagsmEmerald (green fluorescence)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-BRD4
Plasmid#183939PurposeTranscriptional co-activator BRD4 labeled by mCherryDepositorInsertmCherry-BRD4 (Brd4 Synthetic, Mouse)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cerulean-GalT
Plasmid#11930DepositorInsertGalT (GALT Human)
UseTagsExpressionMammalianMutationAmino acids 1-60 of the human galactosyltransfera…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
mApple-M1 Spastin
Plasmid#134461PurposeM1 Spastin expressionDepositorInsertM1 Spastin (SPAST Human)
UseTagsExpressionMammalianMutationMet to Ala change at amino acid 87PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mApple-Sec61b-C1
Plasmid#90993PurposeExpresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cellsDepositorInsertmApple-Sec61b (SEC61B Human, Synthetic)
UseTagsmApple (red fluorescence)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAK-cKal7
Plasmid#145801Purposeexpresses Kalirin-7 with the c-front in mammalian cellsDepositorInsertcKalirin-7 (Kalrn Rat)
UseTagsnoneExpressionMammalianMutationPromoterEIF2aAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98H-pRc/CMV
Plasmid#22044DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationpoint mutant, converting aa 98 from Tyrosine to H…PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCerulean-VSVG
Plasmid#11913DepositorInsertVSVG
UseTagsExpressionMammalianMutationF64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
mApple-M11-92
Plasmid#134466PurposeM11-92 expressionDepositorInsertM1 Spastin Amino Acids 1-92 (SPAST Human)
UseTagsExpressionMammalianMutationM1 Spastin Amino Acids 1-92PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mApple-M1 SpastinE442Q
Plasmid#134464PurposeM1 SpastinE442Q expressionDepositorInsertM1 Spastin (SPAST Human)
UseTagsExpressionMammalianMutationGlu to Gln at amino acid 442PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP
Plasmid#103815PurposeSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue lightDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-mCherry
Plasmid#103816PurposeSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue lightDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SP-His-mCherry-HRP-VhHGFP
Plasmid#112157PurposeExpression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.DepositorInsertanti-GFP FLIPPER-body
UseTagsHis and Thrombin cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP
Plasmid#183926PurposeOptogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP (CRY2 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-NLS
Plasmid#103820PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP-NLS (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-NLS
Plasmid#103819PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-mCherry-NLS (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
mApple-M1 SpastinK388R
Plasmid#134463PurposeM1 SpastinK388R expressionDepositorInsertM1 Spastin (SPAST Human)
UseTagsExpressionMammalianMutationLys to Arg at amino acid 388PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP
Plasmid#103817PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP (CRY2 Mustard Weed)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHR-iRFP713
Plasmid#103818PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-iRFP713 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
Plasmid#217514PurposeEncodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD RecombinaseDepositorInsert10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
Plasmid#217515PurposeEncodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD RecombinaseDepositorInsert13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
Plasmid#217513PurposeEncodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD RecombinaseDepositorInsert10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VP16
Plasmid#183935PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VP16 (UL48 Synthetic)
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIS-rPALcc
Plasmid#145804Purposeexpresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cellsDepositorInsertrat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
UseTagsExpressionMammalianMutationchanged serine 767 to alanine to prevent glycosyl…PromoterCMVAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-M11-92
Plasmid#134467PurposeM11-92 expressionDepositorInsertM1 Spastin Amino Acids 1-92 (SPAST Human)
UseTagsExpressionMammalianMutationM1 Spastin Amino Acids 1-92PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-M11-92
Plasmid#134465PurposeM11-92 expressionDepositorInsertM1 Spastin Amino Acids 1-92 (SPAST Human)
UseTagsExpressionMammalianMutationM1 Spastin Amino Acids 1-92PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
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PHR-GFP-p65
Plasmid#183929PurposeOptogenetic PHR domain coupled to GFP and p65 activation domain; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP-p65 (CRY2 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPObooster
Plasmid#216160PurposePlasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strainsDepositorUseTagsExpressionYeastMutationMKT1 gene containing an aspartic acid to glycine …Promoterendogenous promoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
Plasmid#217510PurposeKD Recombinase dependent conditional smGdP-10XV5 cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
UseTagssmGdP-10XV5ExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-TetR
Plasmid#103813PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL C162F-pRc/CMV
Plasmid#22042DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationpoint mutant, converting aa 162 from Cysteine to …PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL 1-167-pRc/CMV
Plasmid#22078DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationdeletion mutant covering only aa 1-167 of wt VHL …PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL L158S-pRc/CMV
Plasmid#22041DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationpoint mutant converting aa 162 from Cysteine to P…PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
mApple-M1 SpastinHFDD
Plasmid#134462PurposeM1 SpastinHFDD expressionDepositorInsertM1 Spastin (SPAST Human)
UseTagsExpressionMammalianMutationMet to Ala at amino acid 87; His to Asp at amino …PromoterCMVAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL 1-187-pRc/CMV
Plasmid#22079DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationdeletion mutant covering only aa 1-187 of wt VHL …PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
Plasmid#217509PurposeKD Recombinase dependent conditional 1X ALFA cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
UseTagsALFA tagExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-STAT2
Plasmid#183931PurposeOptogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP-STAT2 (CRY2 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-Rta
Plasmid#183930PurposeOptogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP-Rta (CRY2 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-VPR
Plasmid#183928PurposeOptogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP-VPR (CRY2 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-FUSN
Plasmid#183932PurposeOptogenetic PHR domain coupled to GFP and FUSN domain with a high phase separation propensity; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP-FUSN (CRY2 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only