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Plasmid#169247PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.DepositorInsertRiboL1-jGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PSD-95-pTagRFP
Plasmid#52671Purposeexpression of TagRFP-tagged PSD-95DepositorInsertPSD-95 (Dlg4 Rat)
UseTagstagRFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6f
Plasmid#40755PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6f
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMK287 (mAID-Hygro)
Plasmid#72825PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Hygro
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcFL
Plasmid#119877PurposeBacterial Expression construct for protein production and purificationDepositorInsertActivity Regulated Cytoskeletal Protein (Arc Rat)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i1 ONE-GO
Plasmid#189735PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII-WPRE3
Plasmid#165429PurposeExpresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.DepositorInsertFHS-paCaMKII
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianMutationPromoterCaMKII0.4Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
iRFP-PH-PLCdelta1
Plasmid#66841Purposeexpression of near-infrared PI(4,5)P2 biosensorDepositorInsertPH-PLCdelta1
UseTagsiRFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-HaloTag7-LC3
Plasmid#184899PurposeStable expression of HaloTag7-LC3 in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-2dV-Camui (wt)
Plasmid#220366PurposeImproved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)DepositorInsertmVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
UseTagsmEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF HaloTag-SYT1
Plasmid#197280PurposeEncodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTagDepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Grin1 KI
Plasmid#131485PurposeEndogenous tagging of GluN1: N-terminal (amino acid position: A20)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3
Plasmid#165430PurposeExpresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.DepositorInsertFHS-paCaMKII(SD)
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianMutationC450A, L514K, G528A, L531E, N538E: Light-insensit…PromoterCaMKII0.4Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-GFP-LC3-RFP
Plasmid#84573PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-EGFP-NR2a wt
Plasmid#45445DepositorInsertNR2A (Grin2a Rat)
UseTagsEGFPExpressionMammalianMutationEGFP inserted after the predicted signal peptide …PromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-miRFP670nano3
Plasmid#184669PurposeLAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertLAMP1-miRFP670nano3
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHluorin
Plasmid#185537PurposeExpresses LGI1-pHluorin under the CamKII promoterDepositorInsertLGI1 (Lgi1 Rat)
UseLentiviralTagspHluorinExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-rat Nrxn1alpha
Plasmid#58266PurposeExpression plasmid for HA-tagged rat Neurexin 1alphaDepositorInsertNrxn1alpha (Nrxn1 Rat)
UseTagsHAExpressionMammalianMutationsee commentsPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-tdTomato-P2A-paCaMKII
Plasmid#165431PurposeExpresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.DepositorInserttdTomato-P2A-paCaMKII
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFPC2-Gephyrin P1
Plasmid#68815Purposeexpression of fluorescent tagged Geph in mammalian cellsDepositorInsertGephyrin (Gphn Rat)
UseTagsEGFPExpressionMammalianMutationsilent mutations on internal EcoRI site. Gephyri…PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only