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PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV CAG
- Backbone size w/o insert (bp) 4836
- Total vector size (bp) 6624
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Vector typeMammalian Expression, Mouse Targeting, AAV, Cre/Lox, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructions<8 hours
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepAAV CAG iGluSnFR3 v857.GPI
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Alt nameiGluSnFR3 v857.GPI
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Alt nameiGluSnFR3 556dot857.GPI
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Alt namev857.GPI
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SpeciesM. musculus (mouse), R. norvegicus (rat), Synthetic
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Insert Size (bp)1788
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Tags
/ Fusion Proteins
- IgK-chain (N terminal on insert)
- Myc epi tag (C terminal on insert)
- GPI Anchor (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV.CAG.iGluSnFR3.v857.GPI was a gift from Kaspar Podgorski (Addgene plasmid # 178335 ; http://n2t.net/addgene:178335 ; RRID:Addgene_178335) -
For your References section:
Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission. Aggarwal A, Liu R, Chen Y, Ralowicz AJ, Bergerson SJ, Tomaska F, Mohar B, Hanson TL, Hasseman JP, Reep D, Tsegaye G, Yao P, Ji X, Kloos M, Walpita D, Patel R, Mohr MA, Tillberg PW, Looger LL, Marvin JS, Hoppa MB, Konnerth A, Kleinfeld D, Schreiter ER, Podgorski K. Nat Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6. 10.1038/s41592-023-01863-6 PubMed 37142767