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Plasmid#76394Purpose3rd generation lentiviral gRNA plasmid targeting human JAK1DepositorInsertJAK1 (JAK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + S608
Plasmid#58913Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S608 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
JAK1 gRNA (BRDN0001144778)
Plasmid#76393Purpose3rd generation lentiviral gRNA plasmid targeting human JAK1DepositorInsertJAK1 (JAK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-SYMPK
Plasmid#222556PurposePiggyBac transposon plasmid for doxycycline inducible expression of SYMPKDepositorInsertSYMPK (SYMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_CRBN:1-442
Plasmid#211067PurposeMammalian expression of full-length human CRBN the C-terminal NanoLuc luciferase tag.DepositorInsertCRBN:M1-L442 (CRBN Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z
Plasmid#148118PurposeMammalian Expression of HshnRNPE2iso3DepositorInsertHshnRNPE2iso3 (PCBP2 Human)
UseTagsExpressionMammalianMutationone non silent mutation L239 to S compapred to th…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2090
Plasmid#141916PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFANCD2 (FANCD2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CRISPRoff-v2.1
Plasmid#167981PurposeExpresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoterDepositorInsertCRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
UseTags2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-hTERT-IRES-hygro
Plasmid#85140PurposeLentiviral expression of hTERT, used to create immortalized cell linesDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT-IRES-Puro
Plasmid#182590Purposeexpresses TERT in mammalian cellsDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-UbC-FKRP-EF1a-BSD
Plasmid#205150PurposeExpress UbC-driven FKRP and EF-1a driven BSDDepositorInsertFukutin-related protein (FKRP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterUbCAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn
Plasmid#174824PurposeMammalian expression of human MLH1dn (codon optimized)DepositorInserthuman MLH1dn (codon optimized)
UseTagsExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2-P65-HSF1_GFP
Plasmid#61423PurposeExpresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.DepositorInsertMS2(N55K)-P65-HSF1_2A_GFP (HSF1 Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXR001: EF1a-CasRx-2A-EGFP
Plasmid#109049PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targetingDepositorInsertCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationPromoterEF1AAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6HuR
Plasmid#135943PurposeExpresses HuR in E. coliDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_RBFOX2-Halo
Plasmid#175304PurposeProduction of HaloTag fusion proteinDepositorInsertRBFOX2 (RBFOX2 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_MBP_RBFOX2
Plasmid#224292PurposeMBP-RBFOX2 protein purification from bacteriaDepositorInsertRNA binding fox-1 homolog 2 (RBFOX2 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiMPH v2
Plasmid#89308Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.DepositorInsertMS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
UseLentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
TETv4
Plasmid#167983PurposeExpresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoterDepositorInsertTETv4 (TET1CD-XTEN80-dCas9)
UseCRISPR and Synthetic BiologyTags3xNLS, TET1 catalytic domain, and tagBFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD
Plasmid#204359PurposeAAV vector for coexpression of miniDq and KORD under the control of human synapsin promoterDepositorInsertminiDq-mCherry-P2A-HA-KORD
UseAAVTagsHA (for KORD) and mCherry (for miniDq)ExpressionMutationFor miniDq, the third intracellular loop (ICL3) o…PromoterhSynAvailable sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only