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Plasmid#172601PurposeLentiviral plasmid expressing c-terminal V5-tagged GPR3DepositorInsertG protein-Coupled Receptor 3 (GPR3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationWTPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ash1-5p
Plasmid#169712PurposeBacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removedDepositorInsertAsh1 (ASH1 Budding Yeast)
UseTags6xHisExpressionBacterialMutationTruncation including encoding amino acids 420-480ā¦PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_CV2
Plasmid#167165PurposepgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_K90E-IRES_hygro
Plasmid#166407PurposeExpress hPOT1 K90E in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseTagsmycExpressionMammalianMutationK90EPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
MPC027: pAAV-CAG-FLEX-sNovArch-PP-Citrine_KV2.1TS-ER2 (soma targeting)
Plasmid#153196Purposeencodes pAAV soma targeted NovArchDepositorInsertsNovArch
UseAAVTagscitrineExpressionMammalianMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_dead_mt
Plasmid#167270PurposeOverexpression of the dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAP-tag
UseTagsHis x10ExpressionBacterialMutationC145APromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_fast_dead_mt
Plasmid#167272PurposeOverexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
UseTagsHis x10ExpressionBacterialMutationE30R-C145APromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OKMS-KRAB-dCas9
Plasmid#120356PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.DepositorInsertOKMS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [10-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS-KRAB-dCas9
Plasmid#120357PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.DepositorInsertOK+9MS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [1-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:AsCas12a_DR (GB1442)
Plasmid#160574PurposeAsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:AsCas12a_DR
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3566 (pDEST15_Spt6 1223-1451)
Plasmid#91814PurposeBacterial expression of residues 1223-1451 from S. cerevisiae Spt6DepositorInsertSPT6 (SPT6 Budding Yeast)
UseTagsGSTExpressionBacterialMutationdeleted residues 1-1222PromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH1141 (pET151_Spt6 1247-1451)
Plasmid#91815PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6DepositorInsertSPT6 (SPT6 Budding Yeast)
UseTags6xHisExpressionBacterialMutationdeleted residues 1-1246PromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K174A/R179A/K182A
Plasmid#162654PurposeExpresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids K174, R179, and K182 mutated to alaniā¦PromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shNuf2
Plasmid#160961PurposeNuf2 shRNA in pMKO.1 retroviral vectorDepositorInsertNuf2 shRNA (Nuf2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-PIGA
Plasmid#153958PurposeUsed for EGxxFP assay with PIGA intron 5 and exon 6 sequencesDepositorInsertPIGA (a 401-bp fragment from intron 5 and exon 6)
UseA plasmid specific for egxxfp assayTagsN/AExpressionMammalianMutationNo mutationPromoterCAG promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-CD55
Plasmid#153959PurposeUsed for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequencesDepositorInsertCD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
UseA plasmid specific for egxxfp assayTagsN/AExpressionMammalianMutationNo mutationPromoterCAG promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA852 - mec-4p::FynY531F
Plasmid#139209PurposeExpresses activated Fyn kinase in mec-4 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationY531FPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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hnRNPA2_LC_RtoK
Plasmid#139120Purposeexpress hnRNPA2 LC where all R are KDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall R to KPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_NtoS
Plasmid#139121Purposeexpress hnRNPA2 with all N changed to SDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall N to SPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only