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A pentameric protein ring with novel architecture is required for herpesviral packaging.
Didychuk AL, Gates SN, Gardner MR, Strong LM, Martin A, Glaunsinger BA
Elife. 2021 Feb 8;10. pii: 62261. doi: 10.7554/eLife.62261. PubMed Article

Plasmids from Article

ID Plasmid Purpose
162625pcDNA4/TO-2xStrep-ORF68Expresses N-terminal 2x-Strep-tagged ORF68 in mammalian cells
162626pcDNA4/TO-2xStrep-BFLF1Expresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cells
162627pcDNA4/TO-2xStrep-mu68Expresses N-terminal 2x-Strep-tagged mu68 in mammalian cells
162628pcDNA4/TO-2xStrep-UL52Expresses N-terminal 2x-Strep-tagged UL52 in mammalian cells
162629pcDNA4/TO-2xStrep-UL32Expresses N-terminal 2x-Strep-tagged UL32 in mammalian cells
162630pcDNA4/TO-2xStrep-ORF68 C52AExpresses N-terminal 2x-Strep-tagged ORF68 C52A in mammalian cells
162631pcDNA4/TO-2xStrep-ORF68 C373AExpresses N-terminal 2x-Strep-tagged ORF68 C373A in mammalian cells
162632pcDNA4/TO-2xStrep-ORF68 C191A/C192AExpresses N-terminal 2x-Strep-tagged ORF68 C191A/C192A in mammalian cells
162633pcDNA4/TO-2xStrep-ORF68 C415AExpresses N-terminal 2x-Strep-tagged ORF68 C415A in mammalian cells
162634pcDNA4/TO-2xStrep-ORF68 H452AExpresses N-terminal 2x-Strep-tagged ORF68 H452A in mammalian cells
162635pcDNA4/TO-2xStrep-ORF68 C79AExpresses N-terminal 2x-Strep-tagged ORF68 C79A in mammalian cells
162636pcDNA4/TO-2xStrep-ORF68 K435AExpresses N-terminal 2x-Strep-tagged ORF68 K435A in mammalian cells
162637pcDNA4/TO-2xStrep-ORF68 R443AExpresses N-terminal 2x-Strep-tagged ORF68 R443A in mammalian cells
162638pcDNA4/TO-2xStrep-ORF68 K450A/R451AExpresses N-terminal 2x-Strep-tagged ORF68 K450A/R451A in mammalian cells
162639pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182AExpresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells
162640pcDNA4/TO-2xStrep-ORF68 R14AExpresses N-terminal 2x-Strep-tagged ORF68 R14A in mammalian cells
162641pcDNA4/TO-2xStrep-ORF68 K310AExpresses N-terminal 2x-Strep-tagged ORF68 K310A in mammalian cells
162642pcDNA4/TO-2xStrep-ORF68 R14A/K310AExpresses N-terminal 2x-Strep-tagged ORF68 R14A/K310A in mammalian cells
162643pcDNA4/TO-2xStrep-ORF68 K395A/K396AExpresses N-terminal 2x-Strep-tagged ORF68 K395A/K396A in mammalian cells
162644pcDNA4/TO-2xStrep-UL32 C128AExpresses N-terminal 2x-Strep-tagged UL32 C128A in mammalian cells
162645pcDNA4/TO-2xStrep-UL32 C502AExpresses N-terminal 2x-Strep-tagged UL32 C502A in mammalian cells
162646pcDNA4/TO-2xStrep-UL32 C308A/C309AExpresses N-terminal 2x-Strep-tagged UL32 C308A/C309A in mammalian cells
162647pcDNA4/TO-2xStrep-UL32 C544AExpresses N-terminal 2x-Strep-tagged UL32 C544A in mammalian cells
162648pcDNA4/TO-2xStrep-UL32 H581AExpresses N-terminal 2x-Strep-tagged UL32 H581A in mammalian cells
162649pcDNA4/TO-2xStrep-UL32 C155AExpresses N-terminal 2x-Strep-tagged UL32 C155A in mammalian cells
162650pUE1-TSP-ORF68Expresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
162651pUE1-TSP-ORF68 K435AExpresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
162652pUE1-TSP-ORF68 R443AExpresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
162653pUE1-TSP-ORF68 K450A/R451AExpresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
162654pUE1-TSP-ORF68 K174A/R179A/K182AExpresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
162655pUE1-TSP-ORF68 R14A/K310AExpresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
162656pUE1-TSP-ORF68 K395A/K396AExpresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
162657pUE1-TSP-BFLF1Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
162658pUE1-TSP-UL32Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
162659pLJM1-zeo-2xStrep-ORF68Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 in mammalian cells
162660pLJM1-zeo-2xStrep-ORF68 C52ALentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells
162661pLJM1-zeo-2xStrep-BFLF1Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells
162662pLJM1-zeo-2xStrep-mu68Lentiviral vector for stable expression of N-terminal 2xStrep-tagged mu68 in mammalian cells
162663pLJM1-zeo-2xStrep-UL52Lentiviral vector for stable expression of N-terminal 2xStrep-tagged UL52 in mammalian cells
162664pLJM1-zeo-UL32Lentiviral vector for stable expression of UL32 in mammalian cells
166025pCDNA4/TO-ORF68 (tagless)Expresses ORF68 in mammalian cells

Antibodies from Article