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Plasmid#186991PurposeAAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guideDepositorInsertcas7-11-r1045-gggs-r1122, non-targeting crRNA guide
UseAAVTagsExpressionMutationr1045-gggs-r1122 deletionPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-oScarlet
Plasmid#137135PurposeViral expression of oScarletDepositorHas ServiceAAV8InsertoScarlet
UseAAVTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3−3xFLAG−AGO2(D669A)
Plasmid#220461PurposeExpress catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cellsDepositorInsertHuman AGO2 (AGO2 Human)
UseTags3xFLAGExpressionMammalianMutationAspartate 669 to alaninePromoterCMVAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP14-Flag
Plasmid#210346PurposeExpresses SARS-CoV-2 NSP14 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP14 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rosa26_gRNA
Plasmid#196138PurposegRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2DepositorInsertRosa26 gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
shYAP1 # 2
Plasmid#42541DepositorInsertYAP1 (YAP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NRF2-E79Q
Plasmid#207828PurposeFLAG-tagged NRF2-E79Q driven by CMV promoterDepositorInsertNRF2-E79Q (NFE2L2 Human)
UseLentiviralTagsFLAGExpressionMutationE79QPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-FAM208A ts1
Plasmid#115871PurposeFAM208a knockdownDepositorInsertFAM208a shRNA (C3orf63 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF73-2xCSTREP
Plasmid#136232PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF73DepositorInsertORF73
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-G88P3-HA-hM3Dq
Plasmid#213972PurposeAAV vector with G88P3 promoter that can drive robust gene expression in MSNsDepositorInsertG88P3
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5-(SIM)10
Plasmid#126923PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.DepositorInsert5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationinternal BamHI site between (SUMO)5 and (SIM)10PromotertacAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTY-EF1a-wild type mKdm2b-Flag
Plasmid#61739PurposeExpress wild-type mouse histone demethylase Kdm2bDepositorInsertKdm2b (Kdm2b Mouse)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OT1.0
Plasmid#185387PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin sensor GRAB_OT1.0
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVL1393-MAL-SYK
Plasmid#157990PurposeExpresses SYK in insect cell (sf9)DepositorInsertSpleen tyrosine kinase (SYK Human)
UseTagsMet-Ala-Leu and add Leu,Glu residues +His tagExpressionInsectMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
TK-GFP-Nluc-P2A-neo-TK
Plasmid#134896PurposeExpresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locusDepositorInsertTK-GFP-Nluc-P2A-Neo-TK
UseCryptosporidium expressionTagsExpressionMutationPromoterCryptosporidium actin and enolaseAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-FL
Plasmid#128415PurposeExpress INTS3 full-length in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationfull-lengthPromotertac promoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
OR6K6_Deletion_gRNA2
Plasmid#195196Purposedual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFPDepositorInsertOR6K6 dual gRNA (OR6K6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR6K6_Deletion_gRNA1
Plasmid#195195Purposedual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFPDepositorInsertOR6K6 dual gRNA (OR6K6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR6K6_Deletion_gRNA3
Plasmid#195197Purposedual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFPDepositorInsertOR6K6 dual gRNA (OR6K6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-G1-ATP6-Myc-FLAG (puromycin)
Plasmid#86851Purposemammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tagsDepositorInsertATP6 (ATP6 Human)
UseTagsATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…ExpressionMammalianMutationcodon correctedPromoterCMVAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only