-
Plasmid#10771DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutationG129EPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVX puro TRIM21 C54Y - GFP
Plasmid#116942PurposeExpression of TRIM21 C54Y -GFP fusionDepositorInsertTRIM21 C54Y-GFP (TRIM21 Human)
UseLentiviralTagsGFPExpressionMammalianMutationC54YPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-Loop2-8A8G
Plasmid#157983PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (Loop2-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FLAG-NRAS-IRES-Puro
Plasmid#120574PurposeExpresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE3G-BE3-PGK-Puro
Plasmid#110845PurposeLentiviral vector for dox-inducible expression of BE3 (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterTRE3GAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro
Plasmid#129537PurposePiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.DepositorInserttetR-3xFlag-HA-2A-Puro
UseSynthetic BiologyTagsExpressionMutationPromoterhPGK promoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 1
Plasmid#109002PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #1
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-puro-Venus-FLAG-CD59
Plasmid#50377PurposeExpress Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cellsDepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-HA-PIK3CA(H1047R;G320E)
Plasmid#33254DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationchanged histidine 1047 to arginine and glycine 32…PromoterSV40Available sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H2BC11-MMEJ
Plasmid#207760PurposeMMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
attB-mCh-Dnmt3b1-Poly(A)-PuroR
Plasmid#65556PurposePlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsmCherryExpressionMammalianMutationisoform 1PromoterAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-EEA-guide1-PGK-Puro
Plasmid#102904PurposeEBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-guide1-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Puro-T2A-mCitrine-hSmad2-donor
Plasmid#203663PurposeDonor plasmid for CRISPR knock-inDepositorInsertSmad2
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJA285= pSin-TCRab-CMVp-Puro
Plasmid#116875Purposestable TCR expression in human T cellsDepositorUseLentiviralTagsExpressionMammalianMutationdeletion of EF1-a promotorPromoterCMV PromotorAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro human lipin 1-1 shRNA
Plasmid#32019DepositorInsertlipin1 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 Y774F pBabe puro
Plasmid#13599DepositorInsertintegrin beta 6 Y774F (ITGB6 Human)
UseRetroviralTagsExpressionMammalianMutationY774FPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IRES-puro-3xFLAG-NBR1
Plasmid#204546PurposeExpresses 3xFLAG-tagged NBR1 in mammalian cellsDepositorInsertNBR1 (NBR1 Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-2xFLAG-2xSTREP-CCND3-Puro
Plasmid#172622PurposeRetroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cellsDepositorInsertCCND3 (CCND3 Human)
UseRetroviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-HA
Plasmid#162100PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-StrepTagII-HA
UseLentiviralTagsVSVg-StrepTagII-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBMN-puro-P2A-FRB-Fis1
Plasmid#135297Purposestably express FBR-Fis1 in mammalian cells with puromycin selectionDepositorInsertFRB-Fis1 (FIS1 Human)
UseRetroviralTagsFRBExpressionMammalianMutationFis1 tail (aa 93-152)PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro pI3K p110 CAAX
Plasmid#13339DepositorInsertpI3K p110 CAAX (PIK3CA Bovine)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgACSL3.C-Cas9-P2A-Puro
Plasmid#129412PurposeEncoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminusDepositorInsertSpCas9 and sgACSL3.C (ACSL3 Human)
UseCRISPRTagsP2A-PuroExpressionMammalianMutationPromoterhU6Available sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
970 pBabe puroL PTEN R130G
Plasmid#10783DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutationR130GPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-MCS-LexA-VP16-MiniWhite
Plasmid#165898PurposePuromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-LOV
Plasmid#22033PurposeMammalian expression of mVenus tagged LOV2 domainDepositorInsertmVenus-LOV
UseRetroviralTagsExpressionMammalianMutationInsert length based on Venus (720bp) + LOV (450bp)PromoterAvailable sinceSept. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-hGFAP-NEUROD1-T2A-mCherry
Plasmid#178582PurposeExpression of NEUROD1 and mCherry under the human GFAP promoterDepositorInsertNEUROD1 (NEUROD1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-somBiPOLES-mCerulean-IRES-Puro
Plasmid#192585Purposelentiviral vector for bidirectional optogenetic manipulation of Cre-positive neuronsDepositorInsertsomBiPOLES
UseLentiviralTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterEf1-alphaAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRetro-XT-PAK2-CFP(puro)-T402A
Plasmid#81179PurposeRetroviral vector for expression of kinase inactive PAK2 with a CFP fusionDepositorInsertp21 (RAC1) activated kinase 2 (PAK2 Human)
UseRetroviralTagsCFPExpressionMutationT402APromoterP-tight TREAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYJ21-lenti-EF1a-ASAP2f-puro
Plasmid#131065PurposeForced expression of ASAP2fDepositorInsertASAP2f
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-puro-hARAF-shRNA-1
Plasmid#185370PurposeFor tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAFDepositorInsertARAF (ARAF Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras G12V Y40C
Plasmid#12276DepositorInsertH-Ras G12V Y40C (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The Y40C mutat…PromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-Cas9n-RT-T2A-PuroR
Plasmid#172296PurposeIn vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editingDepositorInsertCas9n-RT-T2A-PuroR
UseCRISPRTagsExpressionMutationH840A (Cas9)PromoterT7 promoter A-initiatingAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro/HA-FLAG-RBP2 H483A
Plasmid#14803DepositorInsertRetinoblastoma Binding Protein 2 (KDM5A Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationchanged His 483 to AlaPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
LV UAS-DsRed-Express2-PGK-Puro
Plasmid#190801PurposeLV construct encoding UAS-DsRed-Express2 reporter gene.DepositorInsertUAS-T2A-DsRed-Express2
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)
Plasmid#189955PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK10c
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1
Plasmid#22008PurposeMammalian expression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsmVenusExpressionMammalianMutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSCV N-term HA-FLAG puro BRD7
Plasmid#41934DepositorInsertBRD7 (BRD7 Human)
UseRetroviralTagsHA-FlagExpressionMammalianMutationPromoterAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mycBioID-pBabePuro-myc-BirA*KRASG12V
Plasmid#122211PurposeStably expresses myc-BirA* fused to KRASG12V in mammalian cellsDepositorInsertKRASG12V (KRAS Human)
UseRetroviralTagsmyc-BirA*ExpressionMammalianMutationchanged G12 to VPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
H446 EF1a RA-ABE6.3 p2A Puro
Plasmid#139104PurposeLentiviral expression of ABE6.3.DepositorInsertABE RA6.3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRetro-XT-PAK2-CFP(puro)-L107F
Plasmid#81180PurposeRetroviral vector for expression of kinase active PAK2 with a CFP fusionDepositorInsertp21 (RAC1) activated kinase 2 (PAK2 Human)
UseRetroviralTagsCFPExpressionMutationL107FPromoterP-tight TREAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras G12V E37G
Plasmid#12275DepositorInsertH-Ras G12V E37G (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The E37G mutat…PromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 N35/3NQ
Plasmid#121455Purposeexpression of human PD-L1 mutant in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationN192Q, N200Q and N219QPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-hgp100-PuroR [M1G]
Plasmid#171801PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-hgp100-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B-targeted Aurora B FRET sensor
Plasmid#45232DepositorInsertCENP-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromotert7Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA Oct4A -158
Plasmid#50922PurposeU6 driven sgRNA targeting OCT4 isoform A -158 bp from TSSDepositorInsertOct4A -158 sgRNA (POU5F1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro macroH2A1 shRNA
Plasmid#30517DepositorInsertmH2A1 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABE-2xFLAG-2xSTREP-CCND2-Puro
Plasmid#172628PurposeRetroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cellsDepositorInsertCCND2 (CCND2 Human)
UseRetroviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W
Plasmid#163179PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tagDepositorInsertYAP1 (YAP1 Human)
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MAPKAP1.1-FHH-IRES-Puro
Plasmid#120705PurposeExpresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus productionDepositorInsertMAPKAP1 Isoform 1
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-HA
Plasmid#162109PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to StrepTagII-ProtC-HA
UseLentiviralTagsStrepTagII-ProtC-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only