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hPlekhm1-C/pIRES puro Glue (H987-end)

Plasmid

#73458

Purpose

Express human C-terminal part (H987-end) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-C/pIRES puro Glue (S778-Q986)

Plasmid

#73457

Purpose

Express human C-terminal part (S778-Q986) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-Mid/pIRES puro Glue (Q513-E784)

Plasmid

#73455

Purpose

Express human middle part (Q513-E784) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
RCAS (B) flag Lunatic Fringe (CT#471)

Plasmid

#13980

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Lunatic Fringe (LFNG Chicken)

Use

Retroviral; Avian expression

Tags

Flag

Expression

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
CS2-Myc-mASUN

Plasmid

#47044

Purpose

Expression of tagged-mouse ASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (Asun Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
Cathepsin S S21C/C25A

Plasmid

#84695

Purpose

for expression of mutant Cathepsin S in Leishmania tarantolae

Depositor

Hans Brandstetter

Article

Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

Insert

cathepsin S (CTSS Human)

Use

Expression in leishmania tarantolae

Tags

his-tag

Expression

Mutation

S21C, C25A

Promoter

unknown

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR FOXP3 WWRR

Plasmid

#16364

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Tags

Expression

Bacterial

Mutation

WWRR: T359W, N361W, E399R, E401R

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pLIA CRX EnR (CC#145)

Plasmid

#15155

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

CRX (Crx Mouse)

Use

Retroviral

Tags

Engrailed Repressor

Expression

Mammalian

Mutation

amino acids 7–108, containing the CRX homeobox

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

Plasmid

#69232

Purpose

Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPN1-Gm129

Plasmid

#53765

Purpose

mammalian expression of EGFP-Gm129

Depositor

Aziz Sancar

Article

Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

Insert

Gm129 (Ciart Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

*

Promoter

CMV

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-Akt-AH(R25C)

Plasmid

#39534

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(R25C) (PH domain only) (Akt1 Mouse)

Use

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

PH domain only with R25C mutation

Promoter

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-Cerulean-UCR1

Plasmid

#67578

Purpose

Expresses the UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

Upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Use

Tags

Cerulean and FLAG

Expression

Mammalian

Mutation

Amino acid residues 17-136 of PDE4D3

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
RCAS (A) flag Lunatic Fringe (CT#479)

Plasmid

#13979

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Lunatic Fringe (LFNG Chicken)

Use

Retroviral; Avian expression

Tags

Flag

Expression

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
Djun - antisense

Plasmid

#38338

Depositor

Norbert Perrimon

Article

Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

Insert

djun (Jra Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
mlp-hCHES1sh

Plasmid

#51845

Purpose

shRNA against CHES1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

shRNA against CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pWZL-rCHES1

Plasmid

#51847

Purpose

encodes a CHES1 mRNA that is resistant to shRNA against CHES1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pBS EFEMP1

Plasmid

#19219

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

EFEMP1 (EFEMP1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
p413TEF-CARKL

Plasmid

#51747

Purpose

overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

Depositor

Markus Ralser

Article

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe hygro human Frataxin

Plasmid

#11504

Depositor

Ronald Kahn

Article

Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Frataxin (FXN Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
delta-LIM Isl1

Plasmid

#23261

Depositor

Samuel Pfaff

Article

Thaler et al Cell. 2002 Jul 26. 110(2):237-49.

Insert

Isl1 (Isl1 Rat)

Use

Avian/xenopus/zebrafish

Tags

Lamda-dimer domain

Expression

Mammalian

Mutation

Delta LIM

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Showing: 9461 - 9480 of 18811 results

Results per page:

Sort results by:

hPlekhm1-C/pIRES puro Glue (H987-end)

hPlekhm1-C/pIRES puro Glue (S778-Q986)

hPlekhm1-Mid/pIRES puro Glue (Q513-E784)

RCAS (B) flag Lunatic Fringe (CT#471)

CS2-Myc-mASUN

Cathepsin S S21C/C25A

pENTR FOXP3 WWRR

pLIA CRX EnR (CC#145)

pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

pEGFPN1-Gm129

pEGFP-Akt-AH(R25C)

FLAG-Cerulean-UCR1

RCAS (A) flag Lunatic Fringe (CT#479)

Djun - antisense

mlp-hCHES1sh

pWZL-rCHES1

pBS EFEMP1

p413TEF-CARKL

pBabe hygro human Frataxin

delta-LIM Isl1

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