FLAG-Cerulean-UCR1
(Plasmid
#67578)
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PurposeExpresses the UCR1 region of PDE4D3 in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67578 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFLAG-Cerulean
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUpstream conserved region 1 of phosphodiesterase 4D3
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Alt namePDE4D3 UCR1
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SpeciesH. sapiens (human)
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MutationAmino acid residues 17-136 of PDE4D3
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Entrez GenePDE4D (a.k.a. ACRDYS2, DPDE3, HSPDE4D, PDE43, PDE4DN2, STRK1)
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Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- Cerulean (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-Cerulean-UCR1 was a gift from Graeme Carnegie (Addgene plasmid # 67578 ; http://n2t.net/addgene:67578 ; RRID:Addgene_67578) -
For your References section:
UCR1C is a novel activator of phosphodiesterase 4 (PDE4) long isoforms and attenuates cardiomyocyte hypertrophy. Wang L, Burmeister BT, Johnson KR, Baillie GS, Karginov AV, Skidgel RA, O'Bryan JP, Carnegie GK. Cell Signal. 2015 May;27(5):908-22. doi: 10.1016/j.cellsig.2015.02.003. Epub 2015 Feb 12. 10.1016/j.cellsig.2015.02.003 PubMed 25683917