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pRK-flag-USP19 494-1216 (USP)

Plasmid

#78588

Purpose

Expression flag-USP19 494-1216 (USP) in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1216 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1216 residue…

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4 (E836K)

Plasmid

#29534

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed glutamic acid 836 to lysine

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pAdEasy-EGFP-CLASP2 340-1084

Plasmid

#24524

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1084) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4 (E452K)

Plasmid

#29529

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed glutamic acid 452 to lysine

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pDK480

Plasmid

#134197

Purpose

Mammalian expression of Flip in SKAP and IRES GFP

Depositor

Iain Cheeseman

Article

Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

Insert

untagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Alternate Start, G5 siRNA hardened

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-GFP

Plasmid

#124667

Purpose

Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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RET_Y1062F-SHC

Plasmid

#139620

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET_Y1062F (RET Human)

Use

Tags

StrepIII-HA

Expression

Mammalian

Mutation

Y1062F

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-Fos S374A

Plasmid

#118302

Purpose

Expression of mouse C-Fos

Depositor

Philip Stork

Article

Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

Insert

C-Fos S374A (Fos Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

S374A

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRS316-VTC4

Plasmid

#108691

Purpose

VTC4 sequence expressed in yeast plasmid

Depositor

Michael Downey

Article

Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

Insert

Vacuolar Transporter Chaperone 4

Use

Tags

Expression

Yeast

Mutation

Promoter

VTC4 (native promoter)

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT

Plasmid

#61792

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-3 mutated

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-full length mSesn2 (FL: A+B+C)

Plasmid

#111796

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Full length

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMiR-E6AP-3'UTR-miR-375-mut

Plasmid

#53695

Purpose

Luciferase reporter assay for E6AP 3'UTR with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

UBE3A 3'UTR (UBE3A Human)

Use

Luciferase

Tags

Expression

Mutation

Mutation on miR-375 binding site on E6AP 3'U…

Promoter

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4 (E542K)

Plasmid

#29530

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed glutamic acid 542 to lysine

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-WD40domain

Plasmid

#64678

Purpose

Expresses the WD40 domain of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Wd40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

containing only WD40 domain of WRAP53beta

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
2xHA MED10 pBacPAK8

Plasmid

#17321

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

MED10 (Med10 Mouse)

Use

Tags

2xHA

Expression

Insect

Mutation

Promoter

Available since

March 11, 2008

Availability

Academic Institutions and Nonprofits only
(PM-S608E)FLAG-Cep215-FL

Plasmid

#106906

Purpose

Expresses murine phospho-mimetic CEP215 at S608

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

CEP215 (Cdk5rap2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine-608 to Glutamic Acid

Promoter

CMV

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

Plasmid

#47451

Purpose

AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE BB(N136_0.5NG_C63)-SID4X

Use

AAV; Tale

Tags

2A_phiLOV2.1

Expression

Mammalian

Mutation

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA

Plasmid

#47448

Purpose

AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE BB(N136_0.5NI_C63)-VP64

Use

AAV; Tale

Tags

2A_GFP

Expression

Mammalian

Mutation

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST53-Arl13b20

Plasmid

#40876

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted Arl N-terminal domain; added stop codon

Promoter

CMV

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only
pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

Plasmid

#47449

Purpose

AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE BB(N136_0.5NN_C63)-SID4X

Use

AAV; Tale

Tags

2A_phiLOV2.1

Expression

Mammalian

Mutation

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge