2xHA MED10 pBacPAK8
(Plasmid #17321)

• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Backbone

• Vector backbone: pBacPAK8
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5500
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MED10
• Alt name: Med10
• Species: M. musculus (mouse)
• Insert Size (bp): 500
• Entrez Gene: Med10 (a.k.a. C78613, AA959813, AI385605, D13Wsu50e)
• Tag / Fusion Protein:
  • 2xHA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: Bac 1
• 3′ sequencing primer: Bac 2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Med10 sequence was inserted between XhoI and Kpn1 of pBacPAK8 polylinker after changing the original BacPAK8 BamHI site to NheI. Plasmid can be used for Med10 expression or, by cutting out and replacing Med10 CDS with something else, for construction of BacPAK8 derivatives expressing 2xHA-tagged proteins.