-
Plasmid#108303PurposeExpresses E. coli codon optimized EsCas13d in the pET28a backbone.DepositorInsertEsCas13d
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR2-CFP
Plasmid#13015DepositorInsertToll-Like Receptor 2 (TLR2 Human)
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE4
Plasmid#90338PurposeNRF2 - Antioxidant response element gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNRF2Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST1374-NLS-flag-linker-Cas9
Plasmid#44758DepositorInsertHuman codon optimized Cas9
UseCRISPRTags32 amino acid linker, Flag, and NLSExpressionMammalianMutationcodon-optimizedPromoterCMVAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRE140
Plasmid#118492Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-sgRNA expression vector
Plasmid#51132PurposeFor in vitro transcription of sgRNA from the T7 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterT7Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMP3653
Plasmid#222348PurposeExpression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
HDAC8 Flag
Plasmid#13825DepositorInsertHDAC8 (HDAC8 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 alpha
Plasmid#20351DepositorInsertpDCNA3 Flag p38 alpha (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-Puro
Plasmid#110841PurposeLentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGExpressionMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-spe
Plasmid#117236Purposencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickaseDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-ERCreER-4x6T
Plasmid#196411PurposeAstrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertERT2-Cre-ERT2
UseAAV; Astrocyte-selectiveTagsExpressionMammalianMutationPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
LM627
Plasmid#191551PurposeLM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-hisDepositorInsertccdb
UseTagsSNAC-tag, HIS-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAC435
Plasmid#195238PurposeMammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)DepositorInsertFLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
UseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SELE-Fc(DAPA)-AviTag-6xHis
Plasmid#156908PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSELE (SELE Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TPC1-G-GECO1.2
Plasmid#207143PurposeFusion of Ca2+ reporter and endosomal Ca2+-permeable channelDepositorInsertTPCN1 (TPCN1 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-GCaMP6s
Plasmid#135418PurposeCan be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.DepositorInsertGCaMP6s
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hAtg2A
Plasmid#36456DepositorInsertautophagy-related protein 2 homolog A (ATG2A Human)
UseTagsEGFPExpressionMammalianMutationchanged Proline 656 to ArgininePromoterCMVAvailable sinceMay 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
Halo-Sec31
Plasmid#166895Purposemammalian expression of Sec31 tagged with HaloTagDepositorInsertSec31
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only