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Purposencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117236 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
- Total vector size (bp) 10232
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSelectable Marker: 50 μg/mL spectinomycin in both E.coli and Klebsiella Pneumoniae
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBECKP-spe was a gift from Quanjiang Ji (Addgene plasmid # 117236 ; http://n2t.net/addgene:117236 ; RRID:Addgene_117236) -
For your References section:
Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Wang Y, Wang S, Chen W, Song L, Zhang Y, Shen Z, Yu F, Li M, Ji Q. Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18. 10.1128/AEM.01834-18 PubMed 30217854