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PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
- Total vector size (bp) 11631
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsSelectable Marker: 100 μg/mL hygromycin in both E.coli and Klebsiella Pneumoniae
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCas9
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCasKP-hph was a gift from Quanjiang Ji (Addgene plasmid # 117232 ; http://n2t.net/addgene:117232 ; RRID:Addgene_117232) -
For your References section:
Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Wang Y, Wang S, Chen W, Song L, Zhang Y, Shen Z, Yu F, Li M, Ji Q. Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18. 10.1128/AEM.01834-18 PubMed 30217854