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Showing: 2301 - 2350 of 28566 results

  4. pLenti-CMV-PtenK13E/K289E-Puro

    Plasmid
    #135681
    Purpose
    Constitutive expression (cMV promoter) of K13E/K289E mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    K13E/K289E
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135681-plasmid-map-sequence-id-327600

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  7. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid
    #196196
    Purpose
    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus
    Depositor
    Jürgen Knoblich
    Article
    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75
    Insert
    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    Use
    Homology repair donor
    Tags
    V5-APEX2-mScarlet-I
    Expression
    Mutation
    Promoter
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    196196-plasmid-map-sequence-id-385065

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  9. pIVTR(T7A)-PuroR-T2A-mScarlet

    Plasmid
    #172301
    Purpose
    In vitro transcription of PuroR-T2A-mScarlet synthetic construct
    Depositor
    Patrik Verstreken
    Insert
    PuroR-T2A-mScarlet
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter A-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172301-plasmid-map-sequence-id-340325

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  12. XLone-Puro SPI1 P2A mCherry

    Plasmid
    #140029
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140029-plasmid-map-sequence-id-275844

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  13. PEA1-Puro-HEK3 CTT ins

    Plasmid
    #171996
    Purpose
    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171996-plasmid-map-sequence-id-338997

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  22. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  27. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid
    #68345
    Purpose
    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii
    Inserts
    puromycin
    hCas9
    Use
    CRISPR; Flp/frt
    Tags
    linked to red flouresent protein gene mKateII and…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68345-plasmid-map-sequence-id-438782

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  34. pLenti-EF1a-GFP-miRE_shRen-Puro

    Plasmid
    #135683
    Purpose
    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135683-plasmid-map-sequence-id-327605

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Showing: 2301 - 2350 of 28566 results