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Plasmid#188115PurposeTemplate plasmid to transcribe circular RNA encoding mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-human MYCN
Plasmid#74163PurposeMammalian expression of human MycNDepositorInsertMycN (MYCN Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
evoBxb1_IVT
Plasmid#222341PurposePlasmid for evolved Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved Bxb1
UseTemplate for in vitro transcriptionTagsExpressionMutationPromoterAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60229PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianMutationPromoterCBh and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-scFv-T
Plasmid#67843PurposepET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.DepositorInsertantibody 1D8 light kappa chain
UseTagsHis-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SaPE2
Plasmid#174817PurposeMammalian expression of SaCas9 prime editor 2DepositorInsertSaPE2
UseTagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8s
Plasmid#162371PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8s
UseTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9-CR4
Plasmid#62655PurposeCas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetRDepositorInsertCas9 nuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-HTNC
Plasmid#13763DepositorInsertTAT-NLS-Cre
UseBaculovirusTagsHis Tag, NLS, and TATExpressionBacterialMutationPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
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Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG
Plasmid#176395PurposeExpresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.DepositorInsertGAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a PhoCl-TsCC(B)::KANMX
Plasmid#185906PurposePlasmid for cloning PhoCl fused to TsCC(B) into yeast.DepositorInsertPhoCl-TsCC(B)
UseTagsPhoCl fluorophore with C-terminal TsCC(B)ExpressionYeastMutationPromoterSP6Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e
Plasmid#181751PurposeMMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-BSD
Plasmid#174821PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseTagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.TBG.PI.eGFP.WPRE.bGH
Plasmid#105535PurposeAAV expression of EGFP from TBG promoterDepositorHas ServiceAAV8InsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterTBGAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR4-24XMS2SL-stable
Plasmid#31865DepositorInsert24xMS2 Stem Loops - Stable
UseTagsExpressionMutationPromoterAvailable sinceAug. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
Plasmid#179105PurposepCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINTDepositorInsertpCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
LBR pEGFP-N2 (646)
Plasmid#61996Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertLBR (LBR Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K63
Plasmid#17606PurposeMammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK63 only, other lysines mutated to arginines. Enh…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRT029
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-opti
Plasmid#106280PurposeA version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAS-NanoLuc
Plasmid#87696PurposeGal4VP16 driven expression of NanoLucDepositorInsertNanoLuc
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiSAMPHv2
Plasmid#167934PurposeAll-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCBASceI
Plasmid#26477PurposeI-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI siteDepositorInsertpCBASceI
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Neo DEST (705-1)
Plasmid#17392Purpose3rd gen lentiviral Gateway destination vector, expression, CMV promoter, NeoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
PresentER-Cassette GFP
Plasmid#102942PurposePresentER with removable cassette for cloning of MHC-I antigen (GFP version)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-p15
Plasmid#62656PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmidDepositorInsertexo, beta, gam, sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-His6_pET21a
Plasmid#107178Purposeexpresses His6-tagged miniTurbo in bacteriaDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionBacterialMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-mir-RUP-PheS
Plasmid#44011PurposeLentiviral shRNA vector (pGIPz based) for inducible knockdownDepositorInsertPheS Gly294
UseLentiviralTagsRFPExpressionMammalianMutationPromoterTRE2Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
LCC-ICCG_pET21b
Plasmid#215408PurposeExpression construct for LCC-ICCG gene, codon optimized for expression in E. coliDepositorInsertLCC-ICCG
UseTagsExpressionBacterialMutationF243I/D238C/S283C/Y127GPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMF1706
Plasmid#125582PurposeExpresses peroxisomal roGFP2 (po-roGFP2) in mammalian cellsDepositorInsertpo-roGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-1
Plasmid#36409DepositorTypeEmpty backboneUseFluorescent protein cassette for sub-cloningTagsmEGFPExpressionMutationPromoterno promoterAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
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CaV1.3e[8a,11,31b,Δ32,42a]
Plasmid#49333PurposeRepaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…PromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003
Plasmid#133447PurposeExpresses SpyCatcher003 in bacterial cytoplasmDepositorInsertSpyCatcher003
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Hygro
Plasmid#139462PurposeLentiviral vector with gRNA scaffold and hygromycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: hygroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Hylight
Plasmid#193447PurposeA fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)DepositorInsertHylight Biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
Plasmid#60910PurposedCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag systemDepositorInsertdCas9
UseCRISPR and LentiviralTags2x NLS, HA, and NLSExpressionMammalianMutationPromoterdSV40 PromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-3xFLAG
Plasmid#207684PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPB-CAG-Brainbow3.2-PGK-PuroTK
Plasmid#159928PurposeBrainbow transgenesisDepositorInsertsNFPnls
mOrange2
EGFP
mKate2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP II (pMIG II)
Plasmid#52107Purposea bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
8xGTIIC-luciferase
Plasmid#34615PurposeYAP/TAZ-responsive synthetic promoter driving luciferase expressionDepositorInsertsynthetic TEAD luciferase reporter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-LoxP-DsRed-LoxP-eGFP
Plasmid#65726PurposeFunctions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.DepositorInsertLoxP-DsRed-LoxP-eGFP
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
mRuby3-C1
Plasmid#127808PurposemRuby3 C1 Cloning vectorDepositorInsertmRuby3
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-pAG-Hia5
Plasmid#174373PurposePlasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein AG - hia5
UseTags6xHis and Protein A/GExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCdOpt-BMX
Plasmid#203929PurposeThis plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiaeDepositorInsertBleMX
UseTagsExpressionYeastMutationBleMX was codon optimized for Candida albicans or…PromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOpen-phi29pol
Plasmid#165573PurposeDNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.DepositorInsertphi29 DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.1-TetON-Puro-HIF1a 3809
Plasmid#118705PurposeLentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)DepositorInsertshRNA against human HIF1a
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N-Drd1
Plasmid#104358PurposeExpresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.DepositorInsertDopamine receptor D1 (Drd1) (Drd1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only