pLVX-UbC-rtTA-Ngn2:2A:Ascl1
(Plasmid #127289)

• Purpose: all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)
• Depositing Lab: Fred Gage
• Publication: Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 10.7554/eLife.41356.

Backbone

• Vector backbone: pLVX-tight-puro and pLVX-rtTA(advanced)
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 9766
• Total vector size (bp): 11362
• Modifications to backbone: vector contains both the rtTA(advanced) derived from pLVX-rtTA(advanced) (Clontech) driven by the UbC promoter, as well as Ngn2:2A:Ascl1 driven by the TREtight promoter
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Ngn2:2A:Ascl1
• Alt name: Neurogenin 2, NEUROG2
• Alt name: Mash1
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• GenBank ID: NM_004316.4 NM_024019.4
• Entrez Gene: ASCL1 (a.k.a. ASH1, HASH1, MASH1, bHLHa46)
• Entrez Gene: NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
• Tag / Fusion Protein:
  • Ngn2 and Ascl1 linked via 2A

Gene/Insert 2

• Gene/Insert name: Ngn2:2A:Ascl1
• Alt name: Mash1
• Alt name: Neurogenin 2, NEUROG2
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• GenBank ID: NM_004316.4 NM_024019.4
• Entrez Gene: ASCL1 (a.k.a. ASH1, HASH1, MASH1, bHLHa46)
• Entrez Gene: NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)

Resource Information

• Supplemental Documents:
  • pLVXTP-UNArv.ape
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry