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Purposeall-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127289 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX-tight-puro and pLVX-rtTA(advanced)
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 9766
- Total vector size (bp) 11362
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Modifications to backbonevector contains both the rtTA(advanced) derived from pLVX-rtTA(advanced) (Clontech) driven by the UbC promoter, as well as Ngn2:2A:Ascl1 driven by the TREtight promoter
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameNgn2:2A:Ascl1
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Alt nameNeurogenin 2, NEUROG2
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Alt nameMash1
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SpeciesH. sapiens (human)
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Insert Size (bp)1600
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GenBank IDNM_004316.4 NM_024019.4
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Entrez GeneASCL1 (a.k.a. ASH1, HASH1, MASH1, bHLHa46)
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Entrez GeneNEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
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Tag
/ Fusion Protein
- Ngn2 and Ascl1 linked via 2A
Gene/Insert 2
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-UbC-rtTA-Ngn2:2A:Ascl1 was a gift from Fred Gage (Addgene plasmid # 127289 ; http://n2t.net/addgene:127289 ; RRID:Addgene_127289) -
For your References section:
Chemical modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Herdy J, Schafer S, Kim Y, Ansari Z, Zangwill D, Ku M, Paquola A, Lee H, Mertens J, Gage FH. Elife. 2019 May 17;8. pii: 41356. doi: 10.7554/eLife.41356. 10.7554/eLife.41356 PubMed 31099332