Showing: 9221 - 9240 of 10175 results
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Plasmid#204638PurposeExpression of full-length AsCas12f sgRNA in mammalian cellsDepositorInsertfull-length AsCas12f sgRNA
UseTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
U6-AsCas12f-sgRNA-v2
Plasmid#204639PurposeExpression of trancated AsCas12f sgRNA in mammalian cellsDepositorInserttrancated AsCas12f sgRNA (sgRNA-v2)
UseTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
UCK2_Deletion_gRNA_1
Plasmid#204672PurposeDual gRNA plasmid for UCK2 deletionDepositorInserturidine-cytidine kinase 2 (UCK2 Human)
UseLentiviralTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
UCK2_Deletion_gRNA_2
Plasmid#204673PurposeDual gRNA plasmid for UCK2 deletionDepositorInserturidine-cytidine kinase 2 (UCK2 Human)
UseLentiviralTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pU6-PspCas13b-GEMSgRNA2
Plasmid#204768PurposepU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.DepositorInsertguide RNA targeting GEMS m6A sensor mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pUC57-CmYLCV
Plasmid#205245PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)
Plasmid#205417PurposeMutagenesis of Ntsr1, second guideDepositorInsertNtsr1 (Ntsr1 Mouse)
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pC1.530
Plasmid#205441PurposeLevel 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.DepositorInsertRepB gRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSuperior.retro.puro-sh-γ-tubulin
Plasmid#205602PurposeTet-inducible expression of shRNA targeting gamma-tubulin in human cells, retroviralDepositorInsertsh-g-Tubulin
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCROPseq-VEX-optimized-scaffold
Plasmid#203321PurposeModified CROPseq vector for efficient CRISPR screens in hematopoiesisDepositorInsertsgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSB-BbsI-sgRNA
Plasmid#203359PurposeContains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backboneDepositorInsertBbsI Cut Sites
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRDA_550
Plasmid#203398PurposeAll-in-one lentiviral expression of gRNA and EnAsCas12aDepositorInsertEnhanced Acidaminococcus Cas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR
Plasmid#207355PurposeLentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertRNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)
Plasmid#207356PurposeLentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertN1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)
Plasmid#207357PurposeLentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertL227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Lenti-RNF2-ngRNA+41_EF1a-puroR
Plasmid#207358PurposeLentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.DepositorInsertRNF2 +41 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Lenti-N1303K-ngRNA+14_EF1a-puroR
Plasmid#207359PurposeLentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.DepositorInsertN1303K-CFTR +14 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Lenti-L227R-ngRNA+15_EF1a-puroR
Plasmid#207360PurposeLentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.DepositorInsertL227R-CFTR +15 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pS23.U6<ActB.mus_C-term-KI_gRNA
Plasmid#207408PurposegRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]DepositorInsertActB
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_IL50_HOlocus
Plasmid#194426PurposeExpresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseTagsExpressionYeastMutationNAPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 9221 - 9240 of 10175 results