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pU6-sgGAL4-1

Plasmid

#46915

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-1
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-CYLD-STOP

Plasmid

#60077

Purpose

N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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NgΔIQ FUNgDIQGW

Plasmid

#92232

Purpose

Lentiviral expression of human Ng with deletion mutation

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

Ng (NRGN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

delta IQ

Promoter

hUbC

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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mRFP-mem

Plasmid

#55622

Purpose

This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

mRFP-Mem

Use

Tags

The N-terminal 20 residues of neuromodulin are fu…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Use

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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MEN2A-V5

Plasmid

#139629

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2A (RET Human)

Use

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

C634W

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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MEN2B-V5

Plasmid

#139630

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2B (RET Human)

Use

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

M918T

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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Sa-dCas9-NLS-3xFLAG/pcDNA3.1

Plasmid

#98041

Purpose

Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

Sa-dCas9-NLS-3xFLAG

Use

CRISPR

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

D10A + H557A

Promoter

CMV

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-ARF

Plasmid

#19930

Depositor

Yue Xiong

Article

Zhang et al Cell. 1998 Mar 20. 92(6):725-34.

Insert

cyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

Plasmid

#167268

Purpose

Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HOB

Use

Tags

His x10

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRQ-hRasV12

Plasmid

#18113

Depositor

Alex Swarbrick

Article

Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

Insert

h-RasV12G (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G12V, constitutively active

Promoter

Available since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCAP03-act1p

Plasmid

#120233

Purpose

Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Insert

promoter cassette containing act1p promoter and apramycin resistance gene

Use

Synthetic Biology

Tags

Expression

Mutation

aac(3)IV (apramycin resistance gene)

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-ASCL2

Plasmid

#133375

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

ASCL2 (ASCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆HECT

Plasmid

#81065

Purpose

Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)

Use

Cloning

Tags

Expression

Mutation

C2768A (bp t8302g and g8303c)

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K13R

Plasmid

#63566

Purpose

Expression of human H2A with K13R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 13 is now Arginine

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K15R

Plasmid

#63567

Purpose

Expression of human H2A with K15R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 15 is now Arginine

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pSP64T-hLbCpf1

Plasmid

#107187

Purpose

plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mutation

Promoter

sp6

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET SUMO NL-FKBP-Cas9

Plasmid

#162730

Purpose

Bacterial expression, protein expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

Plasmid

#173873

Purpose

Mammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSNR52-sgTEF1

Plasmid

#46922

Purpose

Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgTEF1 promoter (TEF1 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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