pRQ-hRasV12
(Plasmid
#18113)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 18113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneN/A
-
Backbone manufacturerConstructed from multiple sources
- Backbone size w/o insert (bp) 6664
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)Stbl3
-
Growth instructionsSTBL2 30'C
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameh-RasV12G
-
Alt nameharvey Ras
-
Alt nameoncogenic ras
-
SpeciesH. sapiens (human)
-
Insert Size (bp)569
-
MutationG12V, constitutively active
-
GenBank IDNM_005343
-
Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site Gateway (unknown if destroyed)
- 3′ cloning site gateway (unknown if destroyed)
- 5′ sequencing primer GCT CGT TTA GTG AAC CGT CA (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRQ-hRasV12 was a gift from Alex Swarbrick (Addgene plasmid # 18113 ; http://n2t.net/addgene:18113 ; RRID:Addgene_18113) -
For your References section:
Id1 cooperates with oncogenic Ras to induce metastatic mammary carcinoma by subversion of the cellular senescence response. Swarbrick A, Roy E, Allen T, Bishop JM. Proc Natl Acad Sci U S A. 2008 Apr 8. 105(14):5402-7. 10.1073/pnas.0801505105 PubMed 18378907